Research Highlights

2018

2018

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Exhaustively Trichlorosilylated C1 and C2 Building Blocks: Beyond the Müller-Rochow Direct Process
I. Georg, J. Teichmann, M. Bursch, J. Tillmann, B. Endeward, M. Bolte, H.-W. Lerner, S. Grimme and M. Wagner JACS, 2018, DOI: 10.1021/jacs.8b05950
 
Unexplored nucleotide binding modes for the ABC exporter MsbA
Kaur H, Abreu B, Akhmetzyanov D, Lakatos-Karoly A, Soares CM, Prisner T, Glaubitz C, JACS, 2018, doi: 10.1021/jacs.8b06739
     
 

Correlative single-molecule FRET and DNA-PAINT imaging
N. Deußner-Helfmann, A. Auer, M. Strauss, S. Malkusch, M. Dietz, H.-D. Barth, R. Jungmann* and M. Heilemann* Nano Letters, 2018, published online 26 June 2018. dx.doi.org/10.1021/acs.nanolett.8b02185

  Dynamics of nucleic acids at room temperature revealed by pulsed EPR
M. Gränz, N. Erlenbach, P. Spindler, D. B. Gophane, L. S. Stelzl, S. Th. Sigurdsson & T. F. Prisner: Angewandte Chemie International Edition, 2018,  http://dx.doi.org/10.1002/anie.201803682
     
 

Halogen‐aromatic pi-interactions modulate inhibitor residence time
Heroven C, Georgi V, Ganotra GK, Brennan PE, Wolfreys F, Wade RC, Fernandez-Montalvan AE, Chaikuad A*, Knapp S*: Angewandte Chemie International Edition, 2018, DOI:10.1002/anie.201801666

  Oocyte DNA damage quality control requires consecutive interplay of CHK2 and CK1 to activate p63
M.Tuppi, S. Kehrloesser, D. W. Coutandin, V. Rossi, L. M. Luh, A. Strubel, K. Hötte, M. Hoffmeister, B. Schäfer, T. De Oliveira, F. Greten, E. H. K. Stelzer, S. Knapp, M. De Felici, C. B., F. Gioia Klinger & V. Dötsch: Nature Structural and Molecular Biology, 2018, DOI: 10.1038/s41594-018-0035-7
     
 

Protein aggregation of the p63 transcription factor underlies severe skin fragility in AEC syndrome
C. Russo, C. Osterburg, A. Sirico, D. Antonini, R. Ambrosio, J. M. Würz, J. Rinnenthal, M. Ferniani, S. Kehrloesser, B. Schäfer, P. Güntert, S. Sinha, V. Dötsch und C. Missero: PNAS early edition, 2018, www.pnas.org/cgi/doi/10.1073/pnas.1713773115

   The molecular basis of subtype selectivity of human kinin G-protein-coupled receptors
L. Joedicke*, J. Mao*, G. Kuenze*, C. Reinhart, T. Kalavacherla, H. R A Jonker, C. Richter, H. Schwalbe, J. Meiler, J. Preu, H. Michel* & C. Glaubitz*
Nature Chemical Biology, published online 15.1.2018 (first release) DOI: 10.1038/nchembio.25511
     

2017

2017

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Chromophore Distortions in Photointermediates of Proteorhodopsin Visualized by Dynamic Nuclear Polarization-Enhanced Solid-State NMR
M. Mehler, C.E. Eckert, A.J. Leeder, J. Kaur, T. Fischer, N. Kubatova, L.J. Brown, R.C.D. Brown, J. Becker-Baldus, J. Wachtveitl, C. Glaubitz
J. Am. Chem. Soc., 139 (2017) 16143-16153. DOI: 10.1021/jacs.7b05061
  Structure of the human MHC-I peptide-loading complex
A. Blees, D. Janulienė, T. Hofmann, N. Koller, C. Schmidt, S. Trowitzsch, A. Moeller & R. Tampé
Nature 2017, DOI: 10.1038/nature24627

     
 

Quantitative single-molecule imaging of TLR4 reveals ligand-specific receptor dimerization
C. L. Krüger, M.-T. Zeuner, G. S. Cottrell, D. Widera, M. Heilemann
Science Signaling 2017, DOI: 10.1126/scisignal.aan1308

 

Structure of the TAPBPR–MHC I complex defines the mechanism of peptide loading and editing
C. Thomas, R. Tampé
Science 2017, DOI: 10.1126/science.aao6001

     
 

Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation
H. Steinert, F. Sochor, A. Wacker; J. Buck, C. Helmling, F. Hiller, S. Keyhani, J. Noeske, S. Grimm, M. M Rudolph, H. Keller,  R. A. Mooney, R. Landick, B. Suess, B. Fürtig, J. Wöhnert, H. Schwalbe
eLife 2017, 6:e21297, DOI: 10.7554/eLife.21297

 

Linear ubiquitination of cytosolic Salmonella Typhimurium activates NF-κB and restricts bacterial proliferation
S. J. L. van Wijk, F. Fricke, L. Herhaus, J. Gupta, K. Hötte, F. Pampaloni, P. Grumati, M. Kaulich, Y. Sou, M. Komatsu, F. R. Greten, S. Fulda, M. Heilemann & I. Dikic
Nature Microbiology 2017, doi 10.1038/nmicrobiol.2017.66

     
 

A Vicinal Electrophilic Diborylation Reaction Furnishes Doubly Boron-Doped Polycyclic Aromatic Hydrocarbons
A. John, M. Bolte, H.-W. Lerner, M. Wagner:
Angew. Chem. Int. Ed. 2017, DOI:10.1002/anie.201701591

 

Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation
A. Heuer, M. Gerovac, C. Schmidt, S. Trowitzsch, A. Preis, P. Kötter, O. Berninghausen, T. Becker, R. Beckmann & R. Tampé
Nature Structure and Molecular Biology, doi:10.1038/nsmb.3396

     
 

Engineering fungal de novo fatty acid synthesis for short chain fatty acid production J. Gajewski, R. Pavlovic, M. Fischer, E. Boles & M. Grininger Nature Communications, doi: 10.1038/NCOMMS14650

Engineering fatty acid synthases for directed polyketide production J. Gajewski, F. Buelens, S.Serdjukow, M. Janßen, N. Cortina, H. Grubmüller & M. Grininger Nature Chemical Biology, doi: 10.1038/nchembio.2314

  Fast cAMP Modulation of Neurotransmission via Neuropeptide Signals and Vesicle Loading
W. Steuer Costa, S.-C. Yu, J.F. Liewald, A. Gottschalk
Current Biology, doi: 10.1016/j.cub.2016.12.055

2016

2016

 

Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry
K. Kiosze-Becker, A. Ori, M. Gerovac, A. Heuer, E. Nürenberg-Goloub, U. J. Rashid, T. Becker, R. Beckmann, M. & R.Tampé
Nature Communications, doi: 10.1038/NCOMMS13248

 

Reversible Dihydrogen Activation by Reduced Aryl Boranes as Main-Group Ambiphiles
E. v Grotthuss, M. Diefenbach, M. Bolte, HW Lerner, M. Holthausen, M. Wagner
Angew. Chem. Int. Ed. 2016, DOI: 10.1002/anie.201608324

     
 

Atomic-resolution structure of a disease-relevant Aβ(1-42) amyloid fibril.
Wälti, M. A., Ravotti, F., Arai, H., Glabe, C., Wall, J., Böckmann, A., Güntert, P., Meier, B. H. & Riek, R.
Proceedings of the National Academy of Sciences of the United States of America, 2015, doi: 10.1073/pnas.1600749113

 

Live-cell protein labelling with nanometre precision by cell squeezing
Alina Kollmannsperger, Armon Sharei, Anika Raulf, Mike Heilemann, Robert Langer, Klavs F. Jensen, Ralph Wieneke & Robert Tampé
Nature Cummunications, 2015, 7:10372: doi: 10.1038/ncomms10372

     
   

Megasynthasen: Schlüssel zum Design neuer Antibiotika?
LOEWE Schwerpunkt MegaSyn erforscht Syntheseprinzip riesiger Enzyme/ 4,6 Millionen Euro für vier Jahre

  
     
     
     

2015-2012

2015-2012

2015    
Forschung Frankfurt: Photosynthese mit ultrakurzen Laserpulsen erforschen         

Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFMMoritz Pfreundschuh, David Alsteens, Ralph Wieneke, Cheng Zhang, Shaun R. Coughlin, Robert Tampé, Brian K. Kobilka, Daniel J. Müller
Nature Cummunications, 2015: doi:10.1038/ncomms9857

Arrhythmogenic effects of mutated L-type Ca2+-channels on an optogenetically paced muscular pump in Caenorhabditis elegans
Christina Schüler, Elisabeth Fischer, Lior Shaltiel, Wagner Steuer Costa, Alexander Gottschalk
Nature Scientific Reports, 2015, doi:10.1038/srep14427
  Optogenetic manipulation of cGMP in cells and animals by the tightly light-regulated guanylyl-cyclase opsin CyclOp
Shiqiang Gao, Jatin Nagpal, Martin W. Schneider, Vera Kozjak-Pavlovic, Georg Nagel & Alexander Gottschalk
Nature Communications, 2015, doi: 10.1038/ncomms9046
     
 

SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging
Dr. Ralph Wieneke, Anika Raulf, Alina Kollmannsperger, Prof. Mike Heilemann and Prof. Robert Tampé
Angewandte Chemie International Edition, 2015, doi: 10.1002/anie.201506234

  Boron-Containing PAHs: Facile Synthesis of Stable, Redox-Active Luminophores
M. Sc. Valentin M. Hertz, Dr. Michael Bolte, Dr. Hans-Wolfram Lerner and Prof. Dr. Matthias Wagner
Angewandte Chemie International Edition, 2015, doi: 10.1002/anie.201502977
     

One-Step Synthesis of a [20]Silafullerane with an Endohedral Chloride Ion
Jan Tillmann,Josef Heinrich Wender, Ute Bahr, Michael Bolte, Hans-Wolfram Lerner, Max C. Holthausen and Matthias Wagner
Angewandte Chemie International Edition, 2015, 54, 1-6, DOI: 10.1002/anie.201412050

  Mechanistic insight from the crystal structure of mitochondrial complex I
Volker Zickermann, Christophe Wirth, Hamid Nasiri, Karin Siegmund, Harald Schwalbe, Carola Hunte, Ulrich Brandt (2015)
Science, 2015, Vol. 347 no. 6217 pp. 44-49, DOI: 10.1126/science.1259859
     
2014    
Coupling of remote alternating-access transport mechanisms for protons and substrates in the multidrug efflux pump AcrB.
Eicher, T., Seeger, M. A., Anselmi, C., Zhou, W., Brandstätter, L., Verrey, F., Diederichs, K., Faraldo-Gómez, J. D.*, Pos, K. M.* (2014)
eLife 3:e03145. http://elifesciences.org/content/3/e03145
  Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter
JungMin Kim, Shenping Wu, Thomas M. Tomasiak, Claudia Mergel, Michael B. Winter, Sebastian B. Stiller, Yaneth Robles-Colmanares, Robert M. Stroud, Robert Tampé, Charles S. Craik & Yifan Cheng (2014)
Nature, ahead of print DOI:10.1038/nature13872
     
Revealing the macromolecular targets of complex natural products
Daniel Reker, Anna M. Perna, Tiago Rodrigues, Petra Schneider, Michael Reutlinger, Bettina Mönch, Andreas Koeberle, Christina Lamers, Matthias Gabler, Heinrich Steinmetz, Rolf Müller, Manfred Schubert-Zsilavecz, Oliver Werz & Gisbert Schneider (2014)
Nature Chemistry, 2014, 6, 1072, DOI:10.1038/nchem.2095
 

Confirmed by X-ray Crystallography: The B·B One-Electron s Bond
Alexander Hübner, Andreas M. Diehl, Martin Diefenbach, Burkhard Endeward, Michael Bolte, Hans-Wolfram Lerner, Max C. Holthausen,* and Matthias Wagner* (2014)
Angew Chem Int Ed Engl. Epub ahead of print,  DOI: 10.1002/ange.201402158

     
2013    
Molecular Mechanism of Prion Protein Oligomerization at Atomic Resolution,
Kai Schlepckow, Harald Schwalbe (2013)
Angew Chem Int Ed Engl. Epub ahead of print, DOI: 10.1002/ange.201305184  
  Three-state mechanism couples ligand and temperature sensing in riboswitches,
Anke Reining, Senada Nozinovic, Kai Schlepckow, Florian Buhr, Boris Fürtig and Harald Schwalbe (2013)
Nature, Vol 499, 355-359, DOI: 10.1038/nature12378
     
2012    
Optogenetic Analysis of a Nociceptor Neuron and Network Reveals Ion Channels Acting Downstream of Primary Sensors,
Steven J. Husson, Wagner Steuer Costa, Sebastian Wabnig, Jeffrey N. Stirman, Joseph D. Watson, W. Clay Spencer, Jasper Akerboom, Loren L. Looger, Millet Treinin, David M. Miller, Hang Lu and Alexander Gottschalk (2012)
Current Biology, Epub ahead of print, DOI:10.1016/j.cub.2012.02.066
  Stochastic sensing of proteins with receptor-modified solid-state nanopores,
Ruoshan Wei, Volker Gatterdam, Ralph Wieneke, Robert Tampé & Ulrich Rant (2012)
Nature Nanotechnology, Epub ahead of print, DOI: 10.1038/NNANO.2012.24
     
Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop,
Thomas Eicher, Hi-jea Chaa, Markus A. Seeger, Lorenz Brandstätter, Jasmin El-Delik, Jürgen A. Bohnert, Winfried V. Kern, François Verrey, Markus G. Grütter, Kay Diederichs and Klaas M. Pos (2012)
PNAS early edition, www.pnas.org/cgi/doi/10.1073/pnas.1114944109.
   

2011-2009

2011-2009

2011    
Construction of a Structurally Defined Double-Stranded DNA Catenane
Schmidt TL and Heckel A (2011)
Nano Letters, Epub ahead of print, DOI: 10.1021/nl200303m
          Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS -NMR  [Pressemitteilung]
Ullrich, S.J., Hellmich, U.A., Ullrich, S., Glaubitz, C. (2011)
Nature Chem. Biol. Epub ahead of print, doi:10.1038/nchembio.543  
     
DNA Damage in Oocytes Induces a Switch of the Quality Control Factor TAp63α from Dimer to Tetramer.
G. B. Deutsch, E. M. Zielonka, D. Coutandin, T. A. Weber, B. Schäfer, J. Hannewald, L. M. Luh, F. G. Durst, M. Ibrahim, J. Hoffmann, F. H. Niesen, A. Sentürk, H. Kunkel, B. Brutschy, E. Schleiff, S. Knapp, A. Acker-Palmer, M. Grez, F. McKeon, V. Dötsch
Cell, Vol. 144, 566-576 (2011)
  Ribosome recycling depends on a mechanistic link between the FeS cluster domain and a conformational switch of the twin-ATPase ABCE1.
Barthelme, D., Dinkelaker, S., Albers, S.‐V., Ermler, U. & Tampé, R. (2011)
Proc Natl Acad Sci U S A. (published ahead of print January, 31st)
     
Real-time multimodal optical control of neurons and muscles in freely behaving Caenorhabditis elegans
Jeffrey N Stirman, Matthew M Crane, Steven J Husson, Sebastian Wabnig, Christian Schultheis, Alexander Gottschalk & Hang Lu
Nature Methods, Vol. 8, 153–158 (2011) DOI: doi:10.1038/nmeth.1555
 

Conformation of peptides bound to the transporter associated with antigen processing (TAP)
Herget M, Baldauf C, Schölz C, Parcej D, Wiesmüller KH, Tampé R, Abele R, Bordignon E. (2011)
Proc Natl Acad Sci U S A. (published ahead of print January, 4th; PMID: 21205905)

     
2010    
 Multiplexed Parallel Single Transport Recordings on Nanopore Arrays
Alexander Kleefen, Daniel Pedone, Christian Grunwald, Ruoshan Wei, Matthias Firnkes, Gerhard Abstreiter, Ulrich Rant, and Robert Tampé (2010)
Nano Lett., Articles ASAP (published ahead of print October 27, 2010, DOI: 10.1021/nl1033528)
 

Prediction of blood–brain barrier penetration of poorly soluble drug candidates using surface activity profiling
Anna Christine Petereita, Kelly Swinneyb, Jurgen Menschb, Claire Mackiec, Sigrid Stokbroekxb, Marcus Brewsterb and Jennifer B. Dressman (2010)
European Journal of Pharmaceutics and Biopharmaceutics, Vol. 75, 405-410

     

Structural Studies of 13C,15N-labeled RNA by Solid-State NMR spectroscopy
Alexey V. Cherepanov, Clemens Glaubitz and Harald Schwalbe (2010)
Angew. Chem., published ahead of print June 8, 2010, DOI: 10.1002/anie.200906885

  Single residue within the antigen translocation complex TAP controls the epitope repertoire by stabilizing a receptive conformation
Baldauf C, Schrodt S, Herget M, Koch J, and Tampé R (2010)
Proceedings of the National Academy of Sciences USA (published online May 3rd. doi/10.1073/pnas.1001308107).
In situ assembly of macromolecular complexes triggered by light
Grunwald C, Schulze K, Reichel A, Weiss VU, Piehler J, Wiesmüller KH and Tampé R (2010)
Proceedings of the National Academy of Sciences USA (published online March 1st. doi/10.1073/pnas.0912617107 ).
   
     
2009    
Unfolded-State Structure and Dynamics Influence the Fibril Formation of Human Prion Protein
Christian Gerum, Robert Silvers, Julia Wirmer-Bartoschek, and Harald Schwalbe
Angew. Chem. 2009, 121, 9616 –9620
  Transferrin- and transferrin-receptor-antibody-modified nanoparticles enable drug delivery across the blood-brain barrier (BBB)
Ulbrich, K; Hekmatara, T; Herbert, E; Kreuter, J
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 71, 251-256 (2009)
     
2012    
Bioactivity-guided mapping and navigation of chemical space
S. Renner, W. A L van Otterlo, M. Dominguez Seoane, S. Mücklinghoff, B. Hofmann, S. Wetzel, A. Schuffenhauer, P. Ertl, T. Oprea, D. Steinhilber, L. Brunsveld, D. Rauh & H. Waldmann
Nature Chemical Biology 2009, 5, 585–592
  Protein structure determination in living cells by in-cell NMR spectroscopy
Sakakibara, D., Sasaki, A., Ikeya, T., Hamatsu, J., Hanashima, T., Mishima, M., Yoshimasu, M., Hayashi, N., Mikawa, T., Wälchli, M., Smith, B. O., Shirakawa, M., Güntert, P. & Ito, Y
Nature 458, 102-105 (2009),
[Pressemitteilung]
     
     

2008-2004

2008-2004

2008    
The structure of bradykinin bound to the human GPCR bradykinin-2 as determined by solid-state NMR
Lopez, J.J., Shukla, A.K., Reinhart, C., Schwalbe, H., Michel, H., Glaubitz, C
Angew. Chem. Int. Ed.47, 1668-1671
          Structural basis for the selectivity of the external thioesterase of the surfactin-synthetase
Koglin, A., Löhr, F., Bernhard, F., Rogov, V. R., Frueh, D. P., Strieter, E. R., Mofid, M. R., Güntert, P., Wagner, G., Walsh, C. T., Marahiel, M. A. & Dötsch, V.
Nature 454, 907–911 (2008),
[Pressemitteilung]
     
 2007    
Identification of a lysosomal peptide transport system induced during dendritic cell development
Ö. Demirel, Z. Waibler, U. Kalinke, F. Grünebach, S. Appel, P. Brossart, A. Hasilik, R. Tampé and R. Abele
J. Biol. Chem. 2007; 282; 37836 - 43
  Time-resolved NMR methods resolving ligand-induced RNA folding at atomic resolution
J. Buck, B. Fürtig, J. Noeske, J. Wöhnert, H. Schwalbe 
Proc Natl Acad Sci U S A. 2007;104(40):15699-704
     
Bidirectional optical control of neurons, neural circuits and behaviour in Caenorhabditis elegans
M. Brauner, J. Liewald, A. Gottschalk 
NATURE 2007; 446; 633-639
  Native protein nanolithography that can write, read and erase
A. Tinazli, J. Piehler, M. Beuttler, R. Guckenberger, R. Tampe 
NATURE NANOTECHNOLOGY, 2007;  2 (4): 220-225
     
2006    
Highlights in asymmetric Synthesis and Catalysis
M. Rueping, C. Azap, Angew. Chem. 2006, 118, 7996-7999 (Int. Ed. 45, 7832-7835)
M. Rueping, A. P. Antonchick, T. Theissmann, Angew. Chem. 2006, 118, 6903-6907 (Int. Ed. 45, 6751-6755)
M. Rueping, A. P. Antonchick, T. Theissmann, Angew. Chem. 2006, 118, 3765-3768 (Int. Ed. 45, 3683-3686)
M. Rueping, E. Sugiono, C. Azap, Angew. Chem. 2006, 118, 2679-2681 (Int. Ed. 45, 2617-2619)
Von der Natur lernen (aus Forschung Frankfurt, 2007)
  Molecular phylogeny and evolution of morphology in the social amoebas
Schaap P, Winckler T, Nelson M, Alvarez-Curto E, Elgie B, Hagiwara H, Cavender J, Milano-Curto A, Rozen DE, Dingermann T, Mutzel R, Baldauf SL
Science. 2006; 314(5799); 661-3
     
Localization of multidrug transporter substrates within model membranes
Siarheyeva A, Lopez JJ, Glaubitz C,
Biochemistry 2006; 45(19); 6203-11
  Conformational switches modulate protein interactions in peptide antibiotic synthetases
Koglin A, Mofid MR, Lohr F, Schafer B, Rogov VV, Blum MM, Mittag T, Marahiel MA, Bernhard F, Dotsch V;
Science 2006; 312(5771); 273-6.
     
2005    
Hyperforin activates nonselective cation channels (NSCCs)
Treiber K, Singer A, Henke B, Muller WE;
Br J Pharmacol. 2005; 145(1); 75-83
  Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocytederived dendritic cells
Ralf Gutzmer, Carola Diestel, Susanne Mommert, Brigitta Köther, Holger Stark, Miriam Wittmann, Alexander Kapp and Thomas Werfel;
Journal of Immunology  2005; 174(9); 5224-5232
     
The genome of the social amoeba Dictyostelium discoideum
Nature 2005;  435, 43-57
  Diagnostic tool for the identification of MLL rearrangements including unknown partner genes
Meyer C, Schneider B, Reichel M, Angermueller S, Strehl S, Schnittger S, Schoch C, Jansen MW, van Dongen JJ, Pieters R, Haas OA, Dingermann T, Klingebiel T, Marschalek R;
Proc. Natl. Acad. Sci. USA, 2005, 102(2), 449-454.
     
 2004    
The functional asymmetry of TAP is reflected in the different C-loop sequences
Min Chen, Rupert Abele and Robert Tampé;
Journal of Biological Chemistry, 2004, 279 (44), 46073-46081
  Modulation of Compactness and Long-Range Interactions of Unfolded Lysozyme by Single Point Mutations
Julia Wirmer, Christian Schlörb, Judith Klein-Seetharaman, Ryoma Hirano, Tadashi Ueda, Taiji Imoto, Harald Schwalbe;
Angewandte Chemie (2004) 43, 5780-5785