Arbeitskreis Prof. Dr. Karas


Während die Anwendungen von MALDI und ESI in der Proteinanalytik, aber auch der Analytik von „kleinen Molekülen“, wie z.B. Arzneistoffen und -metaboliten, kontinuierlich wachsen, beschäftigen wir uns mit der Untersuchung der Physik und Chemie der Ionenerzeugung.

Prof. Dr. Michael Karas

Curriculum vitae

Prof. Dr. Michael Karas, born in 1952, studied chemistry at the University of Bonn where he received his PhD in Physical Chemistry in 1982. From 1983 to 1986 he was a postdoc in the group of Franz Hillenkamp at the Institute of Biophysics, University of Frankfurt. In 1987 he joined Franz Hillenkamp at the Institute of Medical Physics and Biophysics within the Medical Faculty at the University of Münster. Starting from 1984, first in Frankfurt and later in Münster, the development of the MALDI technique took place. In 1992, he finalized his Habilitation in Physical Chemistry. He returned to Frankfurt in 1995 as a full professor for Instrumental Analytical Chemistry. In 2001, he switched to the School of Pharmacy and is since then a professor in the Institute of Pharmaceutical Chemistry.

He is a member of ZAFES, a member of the supervisory board of Innovectis and ordinary member of the "Scientific Society on the Johann Wolfgang Goethe-University Frankfurt". He is a member of the editorial boards Journal of Mass Spectrometry and Mass Spectrometry Reviews.

M. Karas  received several scientific awards for the development of the MALDI technique (together with Franz Hillenkamp) : the Mattauch-Herzog award of the Arbeitsgemeinschaft Massenspektrometrie(today DGMS) (1990), the award of the American Society for Mass Spectrometry (ASMS) for "A distinguished Contribution in Mass Spectrometry" (1997), the "Molecular Bioanalytics" award from the GBM (german society for biochemistry and molecular biology) (2000), the ABRF (Association of Biochemical Research Facilities) award for "Recognition of the outstanding contribution to biomolecular technologies" (2003), the Fresenius Award of the GDCh (2003), the Karl-Heinz Beckurts Award (2003), the Torbern Bergman Medal of the Swedish Chemical Society (Analytical Devision) (2006) and the Thomson Medal of the International Mass Spectrometry Society (2006).



  • Ashoub A, Beckhaus T, Berberich T, Karas M, Brüggemann W.
    Comparative analysis of barley leaf proteome as affected by drought stress.

    237 (2013) 771-781
  • Ivashov VA, Grillitsch K, Koefeler H, Leitner E, Baeumlisberger D, Karas M, Daum G.
    Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris.
    Biochim Biophys Acta.
    1831 (2013) 282-90.
  • Sempf K, Arrey T, Gelperina S, Schorge T, Meyer B, Karas M, Kreuter J.
    Adsorption of plasma proteins on uncoated PLGA nanoparticles.

    Eur J Pharm Biopharm
    . 85 (2013) 53-60.
  • Simm S, Papasotiriou DG, Ibrahim M, Leisegang MS, Müller B, Schorge T, Karas M, Mirus O, Sommer MS, Schleiff E
    Defining the core proteome of the chloroplast envelope membranes.
    Front Plant Sci.
    4 (2013) Art. 11.
  • Papasotiriou DG, Markoutsa S, Gorka J, Schleiff E, Karas M, Meyer B.
    MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels.
    Electrophoresis 34 (2013) 2484-94.



  • Meyer B,Wurm JP, Kötter P, Leisegang MS, Schilling V, Buchhaupt M, Held M,Bahr U, Karas M,Heckel A, Bohnsack MT, Wöhnert J, Entian KD.
    The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of {Psi}1191 in yeast 18S rRNA.
    Nucleic Acids Res
    . 39 (2011) 1526-37.
  • Benedikt A,Baltruschat S,Scholz B, Bursen A,Arrey TN,Meyer B, Varagnolo L, Mü ller AM,Karas M,Dingermann T, Marschalek R.
    The leukemogenic AF4–MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures.
    25 (2011) 135-44.
  • Fuchs SW,Jaskolla TW, Bochmann S, Kötter P, Wichelhaus T,Karas M, Stein T, Entian KD.
    Entianin, a Novel Subtilin-Like Lantibiotic from Bacillus subtilis subsp. spizizenii DSM 15029T with High Antimicrobial Activity.
    Appl Environ Microbiol. 77 (2011) 1698-707.
  • Meyer B, Papasotiriou DG, Karas M.
    100% protein sequence coverage: a modern form of surrealism in proteomics.
    Amino Acids
    41 (2011) 291-310.
  • Vettermann C, Castor D, Mekker A, Gerrits B,Karas M, Jäck HM.
    Proteome profiling suggests a pro-inflammatory role for plasma cells through release of high-mobility group box 1 protein.

    11 (2011) 1228-37.
  • Ladig R, Sommer MS, Hahn A, Leisegang MS,Papasotiriou DG, Ibrahim M, Elkehal R,Karas M,Zickermann V, Gutensohn M, Brandt U, Klösgen RB, Schleiff E
    A high-definition native PAGE system for the analysis of membrane complexes.
    Plant J.
    67 (2011) 181-194.
  • Fuchs SW, Proschak A,Jaskolla TW, Karas M, Bode HB.
    Structure elucidation and biosynthesis of lysine-rich cyclic peptides in Xenorhabdus nematophila.
    Org Biomol Chem. 9 (2011) 3130-32.
    Papadioti A, Markoutsa S,Vranakis I, Tselentis Y,Karas M,Psaroulaki A, Tsiotis G.
    A proteomic approach to investigate the differential antigenic profile of two Coxiella burnetii strains.
    J Proteomics 74 (2011) 1150-59.
  • Baeumlisberger D, Rohmer M, Arrey TN, Mueller BF, Beckhaus T, Bahr U,Barka G,Karas M.
    Simple Dual-Spotting Procedure Enhances nLC-MALDI MS/MS Analysis of Digests with Less Specific Enzymes.
    J Proteome Res. 10 (2011) 2889-94.
  • Kühlein HN, Tegeder I, Möser C, Lim HY, Häussler A, Spieth K, Jennes I, Marschalek R,Beckhaus T,Karas M,Fauth M, Ehnert C, Geisslinger G, Niederberger E.
    Nerve injury evoked loss of latexin expression in spinal cord neurons contributes to the development of neuropathic pain.
    PLoS One. 2011 Apr 29;6(4):e19270.
  • Rohmer M, Baeumlisberger D,Stahl B,Bahr U, Karas M.
    Fragmentation of neutral oligosaccharides using the MALDI LTQ Orbitrap.
    Int. J. Mass Spectrom. 305(2011) 199-208.
  • del Pino I, Paarmann I,Karas M, Kilimann MW, Betz H
    The trafficking proteins Vacuolar Protein Sorting 35 and Neurobeachin interact with the glycine receptor b-subunit

    Biochem. Biophys. Res. Comm.
    412 (2011) 435–440.
  • Grillitsch K , Connerth M,  Köfeler H,Arrey TN , Rietschel B, Wagner B,Karas M, Daum G.
    Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: Lipidome meets Proteome

    Biochem. Biophy. Acta
    1811 (2011) 1165-76.
  • Flender C, Wolf C, Leonhard P, Karas M.
    Nano-liquid chromatography-direct electron ionization mass spectrometry: improving performance by a new ion source adapter.

    J. Mass Spectrom.
    46 (2011) 1004-10.
  • Jskolla TW, Karas M.
    Compelling Evidence for Lucky Survivor and Gas Phase Protonation: The Unified MALDI Analyte Protonation Mechanism.
    J. Am. Soc. Mass Spectrom.
    22(2011) 976-88.
  • Svara FN, Kiss A, Jaskolla TW, Karas M, Heeren RM.
    High-Reactivity Matrices Increase the Sensitivity of Matrix Enhanced Secondary Ion Mass Spectrometry.
    Anal Chem.
    83 (2011) 8308-13
  • Arnold A, Arrey TN, Karas M, Persike M.
    Fast quantitative determination of melamine and its derivatives by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Rapid Commun Mass Spectrom.
    25 (2011) 2844-50.

Research areas of the Karas group

150x100 geraet

Proteomics - focus on membrane proteins

Drug Research - Method development

Fundamentals of the ionisation during the MALDI- and ESI-Process


read more here

Overview of our instruments:


Datenbanken (data bases)

ProteinDataBase WWW Home Page 
ExPASy (Swissprot) 
Human Genome Mapping Project 
European Molecular Biology Laboratory (EMBL) 
Jason (Uni Bielefeld) 
The World-Wide Web Virtual Library 
Comp. Biochemistry Research 
Medline (Bibliothek Uni Frankfurt) 
Molecular Biology Databases
Uni Regensburg


Wiley-VCH Journals 
Elsevier Science Home Page 
Book Stacks - Home Page 
Mass Spectrometry Journals
Internet Journal of Science

Massenspektrometrie (mass spectrometry)

Prof. W.-D. Lehmann (in der Zentralen Spektroskopie des  DKFZ Heidelberg)
Prof. B. Spengler, Giessen
Prof. F. Hillenkamp, Muenster
Prof. C.E. Costello, Mass Spectrometry Group, Boston University School of Medicine
Prof. Jean-Claude Tabet (Université P&M Curie, Paris, France)
Mass Spectrometry Groups 
Murray's MS-Page 
MS and Gaseous Ion Chemistry, Rockefeller University 
Mass Spectrometry links (Georgia Institute of Technology)

Chemie (chemistry)

Römpp Chemie Lexikon A-Z
The WWW Chemical Structures Database 
ChemFinder Searching 
GDCh Gesellschaft Deutscher Chemiker 
Chemistry index (FU Berlin) 
Chemikalien: Sicherheitsdaten 
Umrechnung von Einheiten 
American Chemical Society 
Washington University Chemistry Library 


Biochemie (biochemistry)

Dr. Chromo´s Molecular Biology Notebook (beginner´s guide to molecular biology)


Dr. Chromo´s Molecular Biology Notebook (RNA)
(small) RNA database
thermodynamics, software and database of RNA structures
RNA secondary structures (includes other interesting links)
Translation from RNA to Proteins

Methoden und Geräte




340x120 menschen



150x100 paper




Datenbanken, Journals, Massenspektrometrie, Chemie, Biochemie