Arbeitskreis Prof. Dr. Karas

Willkommen

Während die Anwendungen von MALDI und ESI in der Proteinanalytik, aber auch der Analytik von „kleinen Molekülen“, wie z.B. Arzneistoffen und -metaboliten, kontinuierlich wachsen, beschäftigen wir uns mit der Untersuchung der Physik und Chemie der Ionenerzeugung.

Prof. Dr. Michael Karas

Curriculum vitae

Prof. Dr. Michael Karas, born in 1952, studied chemistry at the University of Bonn where he received his PhD in Physical Chemistry in 1982. From 1983 to 1986 he was a postdoc in the group of Franz Hillenkamp at the Institute of Biophysics, University of Frankfurt. In 1987 he joined Franz Hillenkamp at the Institute of Medical Physics and Biophysics within the Medical Faculty at the University of Münster. Starting from 1984, first in Frankfurt and later in Münster, the development of the MALDI technique took place. In 1992, he finalized his Habilitation in Physical Chemistry. He returned to Frankfurt in 1995 as a full professor for Instrumental Analytical Chemistry. In 2001, he switched to the School of Pharmacy and is since then a professor in the Institute of Pharmaceutical Chemistry.

He is a member of ZAFES, a member of the supervisory board of Innovectis and ordinary member of the "Scientific Society on the Johann Wolfgang Goethe-University Frankfurt". He is a member of the editorial boards Journal of Mass Spectrometry and Mass Spectrometry Reviews.

M. Karas received several scientific awards for the development of the MALDI technique (together with Franz Hillenkamp) : the Mattauch-Herzog award of the Arbeitsgemeinschaft Massenspektrometrie(today DGMS) (1990), the award of the American Society for Mass Spectrometry (ASMS) for "A distinguished Contribution in Mass Spectrometry" (1997), the "Molecular Bioanalytics" award from the GBM (german society for biochemistry and molecular biology) (2000), the ABRF (Association of Biochemical Research Facilities) award for "Recognition of the outstanding contribution to biomolecular technologies" (2003), the Fresenius Award of the GDCh (2003), the Karl-Heinz Beckurts Award (2003), the Torbern Bergman Medal of the Swedish Chemical Society (Analytical Devision) (2006) and the Thomson Medal of the International Mass Spectrometry Society (2006).

Publikationen

2013

Ashoub A, Beckhaus T, Berberich T, Karas M, Brüggemann W.
Comparative analysis of barley leaf proteome as affected by drought stress.
Planta 237 (2013) 771-781
Ivashov VA, Grillitsch K, Koefeler H, Leitner E, Baeumlisberger D, Karas M, Daum G.
Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris.
Biochim Biophys Acta. 1831 (2013) 282-90.
Sempf K, Arrey T, Gelperina S, Schorge T, Meyer B, Karas M, Kreuter J.
Adsorption of plasma proteins on uncoated PLGA nanoparticles.
Eur J Pharm Biopharm. 85 (2013) 53-60.
Simm S, Papasotiriou DG, Ibrahim M, Leisegang MS, Müller B, Schorge T, Karas M, Mirus O, Sommer MS, Schleiff E
Defining the core proteome of the chloroplast envelope membranes.
Front Plant Sci. 4 (2013) Art. 11.
Papasotiriou DG, Markoutsa S, Gorka J, Schleiff E, Karas M, Meyer B.
MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels.
Electrophoresis 34 (2013) 2484-94.

2012

Hörnig M, Markoutsa S, Häfner AK, George S, Wisniewska JM, Rödl CB, Hofmann B, Maier T, Karas M, Werz O, Steinhilber D.
Inhibition of 5-lipoxygenase by U73122 is due to covalent binding to cysteine 416
Biochim Biophys Acta. 1821 (2012) 279-86.
Wurm JP, Griese M, Bahr U, Held M, Heckel A, Karas M, Soppa J, Wöhnert J.
Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs.
RNA 18 (2012) 412-20.
Gao Y, Meyer B, Sokolova L, Zwicker K, Karas M, Brutschy B, Peng G, Michel H.
Heme-copper terminal oxidase using both cytochrome c and ubiquinol as electron donors.
Proc Natl Acad Sci U S A. 109 (2012) 3275-80.
Volknandt W, Karas M.
Proteomic analysis of the presynaptic active zone.
Exp Brain Res. 217 (2012) 449-61.
Gorka J, Rohmer M, Bornemann S, Papasotiriou DG, Baeumlisberger D, Arrey TN, Bahr U, Karas M.Perfusion reversed-phase high-performance liquid chromatography for protein separation from detergent-containing solutions: an alternative to gel-based approaches.
Anal Biochem. 424 (2012) 97-107.
Konopka A, Boehm ME, Rohmer M, Baeumlisberger D, Karas M, Lehmann WD.
Improving the precision of quantitative bottom-up proteomics based on stable isotope-labeled proteins.
Anal Bioanal Chem. 404 ( 2012)1079-87
Soltwisch J, Jaskolla TW, Hillenkamp F, Karas M, Dreisewerd K
Ion Yields in UV-MALDI Mass Spectrometry As a Function of Excitation Laser Wavelength and Optical and Physico-Chemical Properties of Classical and Halogen-Substituted MALDI Matrixes
Anal. Chem. 84 (2012) 6567−6576.
Fuchs SW, Sachs CC, Kegler C, Nollmann FI, Karas M, Bode HB
Neutral Loss Fragmentation Pattern Based Screening for Arginine-Rich Natural Products in Xenorhabdus and Photorhabdus
Anal. Chem. 84 (2012) 6948-55.
Gorka J, Bahr U, Karas M.
Graphite supported preparation (GSP) of α-cyano-4-hydroxycinnamic acid (CHCA) for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for peptides and proteins.
J. Am. Soc. Mass Spectrom. 23 (2012) 1949-54.
Winter D, Hung CW, Jaskolla TW, Karas M, Lehmann WD.
Enzyme-cleavable tandem peptides for quantitative studies in MS-based proteomics.
Proteomics 12(2012) 3470-4.

2011

Meyer B,Wurm JP, Kötter P, Leisegang MS, Schilling V, Buchhaupt M, Held M,Bahr U, Karas M,Heckel A, Bohnsack MT, Wöhnert J, Entian KD.
The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of {Psi}1191 in yeast 18S rRNA.
Nucleic Acids Res. 39 (2011) 1526-37.
Benedikt A,Baltruschat S,Scholz B, Bursen A,Arrey TN,Meyer B, Varagnolo L, Mü ller AM,Karas M,Dingermann T, Marschalek R.
The leukemogenic AF4–MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures.
Leukemia 25 (2011) 135-44.
Fuchs SW,Jaskolla TW, Bochmann S, Kötter P, Wichelhaus T,Karas M, Stein T, Entian KD.
Entianin, a Novel Subtilin-Like Lantibiotic from Bacillus subtilis subsp. spizizenii DSM 15029T with High Antimicrobial Activity.
Appl Environ Microbiol. 77 (2011) 1698-707.
Meyer B, Papasotiriou DG, Karas M.
100% protein sequence coverage: a modern form of surrealism in proteomics.
Amino Acids 41 (2011) 291-310.
Vettermann C, Castor D, Mekker A, Gerrits B,Karas M, Jäck HM.
Proteome profiling suggests a pro-inflammatory role for plasma cells through release of high-mobility group box 1 protein.
Proteomics 11 (2011) 1228-37.
Ladig R, Sommer MS, Hahn A, Leisegang MS,Papasotiriou DG, Ibrahim M, Elkehal R,Karas M,Zickermann V, Gutensohn M, Brandt U, Klösgen RB, Schleiff E
A high-definition native PAGE system for the analysis of membrane complexes..
Plant J. 67 (2011) 181-194.
Fuchs SW, Proschak A,Jaskolla TW, Karas M, Bode HB.
Structure elucidation and biosynthesis of lysine-rich cyclic peptides in Xenorhabdus nematophila.
Org Biomol Chem. 9 (2011) 3130-32.
Papadioti A, Markoutsa S,Vranakis I, Tselentis Y,Karas M,Psaroulaki A, Tsiotis G.
A proteomic approach to investigate the differential antigenic profile of two Coxiella burnetii strains.
J Proteomics 74 (2011) 1150-59.
Baeumlisberger D, Rohmer M, Arrey TN, Mueller BF, Beckhaus T, Bahr U,Barka G,Karas M.
Simple Dual-Spotting Procedure Enhances nLC-MALDI MS/MS Analysis of Digests with Less Specific Enzymes.
J Proteome Res. 10 (2011) 2889-94.
Kühlein HN, Tegeder I, Möser C, Lim HY, Häussler A, Spieth K, Jennes I, Marschalek R,Beckhaus T,Karas M,Fauth M, Ehnert C, Geisslinger G, Niederberger E.
Nerve injury evoked loss of latexin expression in spinal cord neurons contributes to the development of neuropathic pain.
PLoS One. 2011 Apr 29;6(4):e19270.
Rohmer M, Baeumlisberger D,Stahl B,Bahr U, Karas M.
Fragmentation of neutral oligosaccharides using the MALDI LTQ Orbitrap.
Int. J. Mass Spectrom. 305(2011) 199-208.
del Pino I, Paarmann I,Karas M, Kilimann MW, Betz H
The trafficking proteins Vacuolar Protein Sorting 35 and Neurobeachin interact with the glycine receptor b-subunit
Biochem. Biophys. Res. Comm. 412 (2011) 435–440.
Grillitsch K , Connerth M, Köfeler H,Arrey TN , Rietschel B, Wagner B,Karas M, Daum G.
Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: Lipidome meets Proteome
Biochem. Biophy. Acta 1811 (2011) 1165-76.
Flender C, Wolf C, Leonhard P, Karas M.
Nano-liquid chromatography-direct electron ionization mass spectrometry: improving performance by a new ion source adapter.
J. Mass Spectrom. 46 (2011) 1004-10.
Jskolla TW, Karas M.
Compelling Evidence for Lucky Survivor and Gas Phase Protonation: The Unified MALDI Analyte Protonation Mechanism.
J. Am. Soc. Mass Spectrom. 22(2011) 976-88.
Svara FN, Kiss A, Jaskolla TW, Karas M, Heeren RM.
High-Reactivity Matrices Increase the Sensitivity of Matrix Enhanced Secondary Ion Mass Spectrometry.
Anal Chem. 83 (2011) 8308-13
Arnold A, Arrey TN, Karas M, Persike M.
Fast quantitative determination of melamine and its derivatives by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 25 (2011) 2844-50.