Prof. Dr. Joachim Engels (†)




  •  Marimani, M. D.; Ely, A.; Buff, M. C. R.; Bernhardt, S.; Engels, J. W.; Scherman, D.; Escriou, V.; Arbuthnot, P.; "Inhibition of replication of hepatitis B virus in transgenic mice following administration of hepatotropic lipoplexes containing guanidinopropyl-modified siRNAs" Journal of Controlled Release 2015, 209, 198-206
  • Ouahrouch, A.; Taourirte, M.; Lazrek, H. B.; Engels, J. W.; Bolte, M.; "Crystal structure of 2-benzamido-N-(2,2-diethoxyethyl)benzamide" Acta Cryst. E 2015, 71(3), o214-o215


  • Rodrigues-Correia, A.; Knapp-Buehle, D.; Engels, J. W.; Heckel, A.; "Selective Uncaging of DNA through Reaction Rate Selectivity" Org. Lett. 2014, 16(19), 5128-5131
  • Ouahrouch, A.; Ighachane, H.; Taourirte, M.; Engels, J. W.; Sedra, M. H.; Lazrek, H. B.; "Benzimidazole-1,2,3-triazole Hybrid Molecules: Synthesis and Evaluation for Antibacterial/Antifungal Activity" Archiv der Pharmazie 2014, 347(10), 748-755
  • Trojanowski, P.; Ploetner, J.; Gruenewald, C.; Graupner, F. F.; Slavov, C.; Reuss, A. J.; Braun, M.; Engels, J. W.; Wachtveitl, J.; "Photo-physical properties of 2-(1-ethynylpyrene)-adenosine: influence of hydrogen bonding on excited state properties" Phys. Chem. Chem. Phys. 2014, 16(27), 13875-13888
  • Ouahrouch, A.; Taourirte, M.; Engels, J. W.; Benjelloun, S.; Lazrek, H. B.; "Synthesis of new 1,2,3-triazol-4-yl-quinazoline nucleoside and acyclonucleoside analogues" Molecules 2014, 19(3), 3638-3653
  • Bats, J. W.; Zivkovic, A.; Parsch, J.; Engels, J. W.; "Three different fluoro- or chloro-substituted 1'-deoxy-1'-phenyl-β-d-ribofuranoses" Acta Cryst. C 2014, 70(4), 400-404
  • Wicke, L., Engels, J. W.; "An unexpected methyl group migration during on-column Stille derivatisation of RNA" Tetrahedron 2014, 70(2), 327-333


  • Wicke, L., Engels, J. W., Gambari, R., Saab, A. M.; "Synthesis and Antiproliferative Activity of Quinolone Nucleosides Against the Human Myelogenous Leukemia K-562 Cell line" Archiv der Pharmazie 2013, 346(10), 757-765
  • Ouahrouch, A., Krim, J., Taourirte, M., Lazrek, H. B., Engels, J. W., Bats, J. W.; "Four related diethyl[(arylamino)(4-ethynylphenyl)methyl]phosphonates" Acta Cryst. C 2013, 69(10), 1157-1163
  • Arbuthnot, P., Hean, J., Ely, A., Marimani, M., Brzezinska, J., D´Onofrio, J., Buff, M. C. R., Engels, J. W., Bernhardt, S.; "Inhibition of viral gene expression" PCT Int. Appl. 2013, WO 2013061295 A1 20130502
  • Bats, J.W.; Schell, P.; Engels, J. W.; "5-[(1R,2R,4R)-2-Methoxy-1,7,7-trimethylbizyclo[2.2.1]hept-2-yl]-tetrazole" Acta Cryst. E 2013, 69(7), o1028
  • Marimani, M.D.; Ely, A.; Buff, M.; Bernhardt, S.; Engels, J. W.; Arbuthnot, P.; "Inhibition of hepatitis C virus replication in cultured cells and in vivo using 2´-O-guanidinopropyl modified siRNAs" Bioorg. Med. Chem. 2013, 21(20), 6145-6155
  • Bats, J.W.; Schell, P.; Engels, J. W.; "Biphenyl- and phenylnaphtaphenyl-substituted 1H-imidazole-4,5-dicarbonitrile catalysts for the coupling of nucleoside methyl-phosphonamidites" Acta Cryst. C 2013, 69(5), 529-533
  • Engels, J. W.; "Gene silencing by chemical modified siRNAs" New Biotechnol. 2013, 30(3), 302-307
  • Gonzalez-Carmona, M.A.; Quasdorff, M.; Vogt, A.; Tamke, A.; Yildis, Y.; Hoffmann, P.; Lehmann, T.; Bartenschlager, R.; Engels, J.W.; "Inhibition of hepatitis C virus RNA translation by antisense bile acid conjugated phosphorothioate modified oligodeoxynucleotides (ODN)" Antivir. Res. 2013, 97(1), 49-59
  • Robaldo, L.; Pontiggia, R.; Di Lella, S.; Estrin, D.; Engels, J.W.; Iribarren, A.M.; Montserrat, J.M.; "Conformational States of 2´-C-Methylpyrimidine Nucleosides in Single and Double Nucleic Acid Stranded Structures" J. Phys. Chem. B  2013, 117(1), 57-69
  • Krim, J.; Taourirte, M.; Gruenewald, C.; Krstic, I.; Engels, J. W.; "Microwave assisted click chemistry for nucleoside functionalization: usetul derivatives for analytical and biological applications" Synthesis 2013, 45(3), 396-405


  • Gohlke, H.; Bozilovic, J.; Engels, J.W.; "Synthesis and properties of fluorinated nucleobases in DNA an RNA" Molecular Medicine and Medicinal Chemistry 2012, 3-32
  • Brzezinska, J.; D'Onofrio, J.; Buff, M. C. R.; Hean, J.; Ely, A.; Marimani, M.; Arbuthnot, P.; Engels, J. W.; "Synthesis of 2'-O-guanidinopropyl-modified nucleoside phosphoramidites and their incorporation into siRNAs targeting hepatitis B virus" Bioorg. Med. Chem. 2012, 20(4), 1594-1606
  • Wicke, L.; Engels, J. W.; "Postsynthetic on Column RNA Labeling via Stille Coupling" Bioconjugate Chem. 2012, 23(3), 627-642
  • Engels, J. W.; "Medicinal Chemistry of Nucleic Acids" ChemMedChem 2012, 7(6), 1124-1125
  • Ouahrouch, A.; Taourirte, M.; Lazrek, H. B.; Bats, J. W.; Engels, J. W.; "2-({4-[4-(1H-Benzimidazol-2-yl)phenyl]-1H-1,2,3-triazol-1-yl}methoxy)ethanol" Acta Cryst. E 2012, E68(6), o1908
  • Krim, J.; Taourirte, M.; Engels, J. W.; "Synthesis of 1,4-disubstituted mono- and bis-triazolocarbo-acyclonucleoside analogues of 9-(4-hydroxybutyl)guanine by Cu(I)-catalyzed click azide-alkyne cycloaddition" Molecules 2012, 17, 179-190
  • Krim, J.; Gruenewald, C.; Taourirte, M.; Engels, J. W.; "Efficient microwave-assisted synthesis of 5-benzimidazolyl-2´-deoxyuridines" Bioorg. Med. Chem. 2012, 20(1), 480-486


  •  Krstic, I.; Hänsel, R.; Romainczyk, O.; Engels, J. W.; Dötsch; V.; Prisner, T. F.; "Long-Range Distance Measurement on Nucleic Acids in Cells by Pulsed EPR Spectroscopy" Angew. Chem. Int. Ed. 2011, 50(22), 5070-5074.
  • Romainczyk, O.; Endeward, B.; Prisner, T. F.; Engels, J. W.; "The RNA-DNA hybrid structure determined by EPR, CD and RNase H1" Mol. Biosyst. 2011, 7(4), 1050-1052
  • Knapp, D. C.; Serva, S.; D´Onofrio, J.; Keller, A.; Lubys, A.; Kurg, A.; Remm, M.; Engels, J. W.; "Fluoride-Cleavable, Fluorescently Labelled Reversible Terminators: Synthesis and Use in Primer Extension" Chem. Eur. J. 2011, 17(10), 2903-2915
  • Krim, J.; Sillahi, B.; Taourirte, M.; Eddarir, S.; Engels, J. W.; "Synthesis and antibacterial activity of unsaturated pyrimidine carboacyclonucleoside analogues" Coll. Czech. Chem. Commun. 2011, 76(11), 1299-1305
  • Sokocevic, A.; Han, S.; Engels, J. W.; "Biophysical characterization of α-amylase inhibitor Parvulustat (Z-2685) and comparison with Tendamistat" Biochim. Biophya. Acta 2011, 1814(10), 1383-1393


  •  Engels, J. W.; Odadzic, D.; Smicius, R.; Haas, J. "Chemical synthesis of 2'-O-alkylated siRNAs" Methods in Molecular Biology 2010, 623(RNA Interference), 155-170.
  • Knapp, D. C.; D'Onofrio, J.; Engels, J. W. "Fluorescent Labeling of (Oligo)Nucleotides by a New Fluoride Cleavable Linker Capable of Versatile Attachment Modes" Bioconjugate Chem. 2010, 21(6), 1043-1055.
  • Koller, A. N.; Bozilovic, J.; Engels, J. W.; Gohlke, H. "Aromatic N versus aromatic F: bioisosterism discovered in RNA base pairing interactions leads to a novel class of universal base analogs" Nucleic Acids Res, 2010, 38(9), 3133-3146.
  • Pontiggia, R.; Pontiggia, O.; Simian, M.; Montserrat, J. M.; Engels, J. W.; Iribarren, A. M. "2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor" Bioorg. Med. Chem. Lett. 2010, 20(9), 2806-2808.
  • Wurm, J. P.; Meyer, B.; Bahr, U.; Held, M.; Frolow, O.; Koetter, P.; Engels, J. W.; Heckel, A.; Karas, M.; Entian, K.-D.; Woehnert, J. "The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase" Nucleic Acids Res. 2010, 38(7), 2387-2398.
  • Foerster, U.; Lommel, K.; Sauter, D.; Gruenewald, C.; Engels, J. W.; Wachtveitl, J. "2-(1-Ethynylpyrene)-adenosine as a folding probe for RNA-pyrene in or out" ChemBioChem 2010, 11(5), 664-672.
  • Krstic, I.; Frolow, O.; Sezer, D.; Endeward, B.; Weigand, J. E.; Suess, B.; Engels, J. W.; Prisner, T. F. "PELDOR Spectroscopy Reveals Preorganization of the Neomycin-Responsive Riboswitch Tertiary Structure" J. Am. Chem. Soc. 2010, 132(5), 1454-1455.
  • Bramsen, J.B.; Pakula, M.M.; Hansen, T.B.; Bus, C.; Kangkjaer, N.; Odadzic, D.; Smicius, R.; Wengel, S.L.; Chattopadahyaya, J.; Engels, J.W.; Herdewijn, P.; Wengel, J.; Kjems, J. "A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects" Nucleic Acids Res.. 2010, 38(17), 5761-5773.
  • Foerster, U.; Gruenewald, C.; Engels, J. W.; Wachtveitl, J. "Ultrafast Dynamics of 1-Ethynylpyrene-Modified RNA: A Photophysical Probe of Intercalation" J. Phys. Chem. B 2010, 114(35), 11638-11645.


  • Förster, U.; Gildenhoff, N.; Grünewald, C.; Engels, J.W.; Wachtveitl, J. „Photophysics of 1‑ethynylpyrene-modified RNA base adenine“ J. Lumin. 2009, 129, 1454-1458.
  • Müller-Kuller, T.; Capalbo, G.; Klebba, C.; Engels, J.W.; Klein, S.A. „Identification and Characterization of A Highly Efficient Anti-HIV Pol Hammerhead Ribozyme” Oligonucleotides 2009, 19(3), 265-271.
  • Frolow, O.; Bats, J.W.; Engels, J.W. “3-Ethynyl-2,2,5,5-tetramethyl-1-oxyl-3-pyrroline” Acta Cryst. 2009, E65, o1848.
  • Bramsen, J.B.; Laursen, M.B.; Nielsen, A.F.; Hansen, T.B.; Bus, C.; Langkjær, N.; Babu, B.R.; Højland, T.; Abramov, M.; Aerschot, A.V.; Odadzic, D.; Smicius, R.; Haas, J.; Andree, C.; Barman, J.; Wenska, M.; Srivastava, P.; Zhou, C.; Honcharenko, D.; Hess, S.; Müller, E.; Bobkov, G.V.; Mikhailov, S.N.; Fava, E.; Meyer, T.F.; Chattopadhyaya, J.; Zerial, M.; Engels, J.W.; Herdewijn, P.; Wengel, J.; Kjems, J. “A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity” Nucleic Acids Res. 2009, 37(9), 2867-2881.
  • Keller, A.C.; Serva, S.; Knapp, D.C.; Kwiatkowski, M.; Engels, J.W. „Synthesis of 3´-O-(2-cyanoethyl)-2´-deoxythymidine-5´-phosphate as a model compound for evaluation of cyanoethyl cleavage” Collect. Czech. Chem. Commun. 2009, 74(4), 515-534.
  • Bats, J.W.; Frolow, O.; Engels, J.W. “4-Ethynyl-2,2,6,6-tetramethyl-1,2,5,6-tetrahydropyridine-N-oxide” Acta Cryst. 2009, E65, o529.
  • Rehm, S.; Han, S.; Hassani, I.; Sokocevic, A.; Jonker, H.R.A.; Engels, J.W.; Schwalbe, H. „The High Resolution NMR Structure of Parvulustat (Z-2685) from Streptomyces parvulus FH-1641: Comparison with Tendamistat from Streptomyces tendae 4158” ChemBioChem 2009, 10(1), 119-127.
  • Krim, J.; Sillahi, B.; Taourirte, M.; Rakib, E. M.; Engels, J. W. "Microwave-assisted click chemistry: synthesis of mono and bis-1,2,3-triazole acyclonucleoside analogues of Acyclovir via copper(I)-catalyzed cycloaddition" ARKIVOC, 2009, (13), 142-152.


  • Kopitz, H.; Zivkovic, A.; Engels, J.W. “Determinants of the Unexpected Stability of RNA Fluorobenzene Self Pairs” ChemBioChem 2008, 9(16), 2619-2622
  • Frolow, O.; Engels, J.W. “TPA labeled oligonucleotides for long range distance measurements by EPR” Nucl. Acids Symp. Ser. 2008, 10, 343-344
  • Keller, A.; Engels, J.W. “Synthesis of 3´-O-cyanoethyl-2´-deoxythymidine-5´-monophosphate and its use for the sequencing-by-synthesis technology” Nucl. Acids Symp. Ser. 2008, 10, 376-377
  • Wicke, L.; Engels, J.W. „Synthesis of 6-substituted quinolone nucleosides as potential HIV integrase inhibitors“ Nucl. Acids Symp. Ser. 2008, 10, 473-475
  • Smicius, R., Engels, J.W. “Preparation of Zwitterionic Ribonucleoside Phosphoramidites for Solid-Phase siRNA Synthesis“ J. Org. Chem. 2008, 73, 4994-5002.
  • Grünewald, C., Kwon, T., Piton, N., Förster, U., Wachtveitl, J., Engels, J.W. "RNA as scaffold for pyrene excited complexes“ Bioorg. Med. Chem2008, 16 (1), 19-26.
  • Odadzic, D., Bramsen, J. B., Smicius, R.,  Bus, C.,  Kjems J., Engels, J.W. “Synthesis of 2´-O-modified Adenosine Building Blocks and application for RNA Interference” Bioorg. Med. Chem. 2008, 16 (1), 518-529. 


  • Haas, J., Engels, J. W.  "A novel entry to 2´-O-aminopropyl modified nucleosides amenable for further modifications", Tetrahedron Lett.  2007, 48 (50), 8891-8894.
  • Nikolaus, N.,  Bozilovic, J.,  Engels, J.W. “Microwave-assisted Vorbrüggen Ribosylation reaction” Nucleosides, Nucleotides & Nucleic Acids  2007, 26 (8-9), 889-892.
  • Bozilovic J., Engels, J.W. “Synthesis of Fluorinated Indoles as RNA Analogues” Nucleosides, Nucleotides & Nucleic Acids  2007, 26 (8-9), 869-871.
  • Haas, J., Mueller-Kuller, T., Klein, S., Engels, J. W. "RNAi activity of siRNAs modified with 2’-Aminoalkyl-Substituted Fluorinated Nucleobases" Nucleosides, Nucleotides & Nucleic Acids; 2007, 26 (6), 865-868.
  • Kwon, T.,  Piton, N.,  Grünewald, C., Engels, J.W. “Synthesis of Pyrene Labeled RNA for Fluorescence Measurements” Nucleosides, Nucleotides & Nucleic Acids  2007, 26 (10),1381-6.
  • Odadzic, D. and Engels, J. W. "Different strategies for the synthesis of 2'-O-aminothyl adenosine building blocks" Nucleosides, Nucleotides & Nucleic Acids; 2007, 26 (8-9), 873-877.
  • Frolow, O., Bode, B.E. and Engels, J. W. "The Synthesis of EPR Differentiable Spinlabels and Their Coupling to Uridine" Nucleosides, Nucleotides & Nucleic Acids; 2007, 26 (6),655-659.
  • Bozilovic, J.; Bats, J.; Engels, J.W. “Synthesis and structure of fluoroindole nucleosides“, Can. J. Chem2007, 85 (4), 283-292.
  • A. Földesi;  A. Keller;  A. Stura;  S. Zigmantas;  M. Kwiatkowski;  D. Knapp; J. W. Engels, “The Fluoride Cleavable 2-(Cyanoethoxy)Methyl (CEM) Group as Reversible 3'-O-Terminator for DNA Sequencing-by-Synthesis - Synthesis, Incorporation, and Cleavage”, Nucleosides, Nucleotides & Nucleic Acids, 2007, 26, 271-275.
  • Wittel, G.; Bats, J.; Engels, J.W. “3’,5’-Bis(tert-butyldimethylsilyl)-5-iodo-2’-deoxycytidine“, Acta Cryst. 2007, E63, o2657-o2659.
  • Beckmann, J.; Kincaid, K.; Hocek, M.; Spratt, T.; Engels, J.W.; Cosstick, R.; and Kuchta, R.D., “Human DNA Polymerase a Uses a Combination of Positive and Negative Selectivity To Polymerize Purine dNTPs with High Fidelity”, Biochemistry, 2007, 46, 448-460.
  • Piton, N.; Mu, Y.; Stock, G.; Schiemann, O. and Engels, J.W., “Base-specific spin-labeling of RNA for structure determination”, Nucleic Acids Res., 2007, 35, 3128-3143.
  • Schiemann, O.; Piton, N.; Plackmeyer, J.; Bode, B.; Prisner, T. and Engels, J.W., “Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distance”, Nature Protocols, 2007, 2, (4), 904-923. 


  • Adams, M.; Bats, J., Nikolaus, N., Engels, J.W., “Microwave-assisted synthesis of fluoroquinolones and their nucleosides as inhibitors of HIV integrase” Coll. Czech. Chem. Commun. 2006, 71, 978-990.


  • Engels, J.W., “Gensynthese auf Mikrochips”, Angew. Chem. 2005, 117, 7328-7331.
  • Hofmann, T.; Zweig, K.; Engels, J.W., “A new synthetic approach for the synthesis of N2-modified guanosines”, Synthesis, 2005, 11, 1797-1800.
  • Kincaid, K.; Beckmann, J.; Zivkovic, A.; Halcomb, R.L.; Engels, J.W.; Kuchta, R.D. “Exploration of factors driving incorporation of unnatural dNTPS into DNA by Klenow fragment (DNA polymerase I) and DNA polymerase a”, Nucleic Acids Res., 2005, 38 (8), 2620-2628.
  • Couvreur, P.; Braguer, D.; Crommelin, D. J. A.; Duchene, D.; Engels, J. W.; Kerr, D. J.; Krogsgaard-Larsen, P.; Meijer, D. K.; Paoletti, R.;Wagner, E. "Contribution of academic research to discovery and development of medicines: current status and future opportunities" EUFEPS 2005, 24, 245-252.
  • Taourirte M., Lazrek H.B., Vasseur J.J., Engels, J.W., “Homo and Heterodimers of DDI, D4T and AZT: Influence of (5’-5’) Thiolcarbonate-carbamte linkage on anti-HIV acctivity”, Nucleosides, Nucleotides & Nucleic Acids, 2005, 24, 523-525.
  • Bozilovic, J., Bats, J.W., Engels, J.W., “Synthesis and Crystal Structures of Fluorinated Indoles as RNA Analogues” Collection Symposium Series 2005, 7, 385-386.
  • Zivkovic, A.; Engels, J. W. “RNA recognition by Fluor-Aromatic substituted”, Nucleosides, Nucleotides & Nucleic Acids, 2005, 24, 1023-1027.
  • Klöpffer, A. E.; Engels, J. W. “Synthesis and purification of fluorinated Benzimidazole and Benzene Nucleoside-5’-Triphosphates, Nucleosides, Nucleotides & Nucleic Acids, 2005, 24, 651-654.
  • Piton, N.; Engels, J. W. “Synthesis of Spin-labeled RNAS for long range distance measurements by PELDOR”, Nucleosides, Nucleotides & Nucleic Acids, 2005, 24, 771-775. 


  • Zacharias, M.; Engels, J. W. "Influence of a fluorobenzene nucleobase analogue on the conformational flexibility of RNA studied by molecular dynamics simulations", Nucleic Acids Res. 2004, 32 (21), 6304-6311
  • Moore, C. L.; Zivkovic, A.; Engels, J. W.; Kuchta, R. D. "Human DNA Primase Uses Watson-Crick Hydrogen Bonds To Distinguish between Correct and Incorrect Nucleoside Triphosphates" Biochemistry, 2004, 43 (38), 12367-12374
  • Jahn-Hofmann, K. , Engels, J. W. "Efficient Solid Phase Synthesis of Cleavable Oligodeoxy-nucleotides Based on a Novel Strategy for the Synthesis of 5´-S-(4,4´-Dimethoxytrityl)-2´-deoxy-5´-thionucleoside Phosphoramidites" Helvetica Chim. Acta, 2004, 87 (11), 2812-2828
  • Engels, J. W.; Parsch, J. "Nucleic Acid Drugs"  in Molecular Biology in Medicinal Chemistry, Wiley-VCH, Hrsg.: Dingermann, T.; Steinhilber, D.; Folkers, G.; Weinheim, 2004, 153-178.
  • Klöpffer, A.E.; Engels, J.W. “Synthesis of 2´-Aminoalkyl Substituted Fluorinated Nucleobases and Their Influence on the Kinetic Properties of Hammerhead Ribozymes” ChemBioChem, 2004, (5), 100-109
  • Schiemann, O.; Piton, N.; Mu, Y.; Stock, G.; Engels, J.; Prisner, T. “A PELDOR Based Nanometer Distance Ruler for Oligonucleotides” J .Am. Chem. Soc., 2004, 126, 5722


  •  Rochdi, A.; Taourirte, M.; Redwane, N.; Sebti, S.; Engels, J.W.; Lazrek, H.B. „Doped Natural Phosphate: A New and Evironmentally Friendly Catalyst in Nucleoside Synthesis” Nucoleosides, Nucleotides, 2003, 22(5-8), 1167-1170
  • Ait Mohamed, L.; Taourirte, M.; Rochdi, A.; Lazrek, H.B.; Vasseur, J.J.; Engels, J.W.; Pannecouque, C.; DeClercq, E. „Synthesis of New Homo- and Heterodimers of 2´,3´-Dideoxyinosine(ddi) Using Ester Linkages“ Nucoleosides, Nucleotides 2003, 22(5-8), 829-831
  • Alahiane, A.; Taourirte, M.; Rochdi, A; Redwane, N.; Sebti, S.; Engels, J.W.; Lazrek, H.B. „Building Blocks for Polyamide Nucleic Acids: Facile Synthesis Using Potassium Fluoride Doped Natural Phosphate as Basic Catalyst“ Nucoleosides, Nucleotides 2003, 22(2), 109-114
  • G. Pappenberger, A. Bachmann, R. Müller, H. Aygün, J. W. Engels, T. Kiefhaber, "Kinetic Mechanism and Catalysis of a Native-state Prolyl Isomerization Reaction" J. Mol. Biol. 2003, 326, 235-246
  • F. Eckstein, J. W. Engels, “Antisense Oligonucleotides as Potential Drugs” in "Encyclopedia of Molecular Cell Biology and Molecular Medicine", 2003, Vol.1, Wiley-VCH, 403-414.
  • N. Piton, J. W. Engels, "Determination of RNA Tertiary Structure by Electron Paramagnetic Resonance Spectroscopy (EPR)" Nucleosides, Nucleotides & Nucleic Acids, 2003, 22, 5-8, 1161-1164
  • A. E. Klöpffer, J. W. Engels, "The Effect of Universal Fluorinated Nucleobases on the Catalytic Activity of Ribozymes" Nucleosides, Nucleotides & Nucleic Acids 2003, 22, 5-8, 1347-1350
  • A. Zivkovic, J. W. Engels, "Synthesis of Modified RNA-Oligonucleotides for Structural Investigations" Nucleosides, Nucleotides & Nucleic Acids 2003, 22, 5-8, 1167-1170


  • K. Heermeier, H. Prydz, J. Reinhard-Rupp, J. W. Engels, "Meeting Review: ESF Workshop on ´Impact of Nucleic Acid Chemistry on Gene Function Analysis: Antisense, Aptamers, Ribozymes and RNAi´ " Comp. Funct. Genom, 2002, 3, 441-446
  • A. Zivkovic, J. W. Engels, "Crystal Structures of Fluoro- and Chloro-Modified Benzene Nucleosides" Coll. Symp. Series, 2002, 5, 396-398
  • N. Piton, F. Godde, J. W. Engels, "Determination of RNA Tertiary Structure by Advanced Electron Paramagnetic Resonance Spectroscopy (EPR)" Coll. Symp. Series 2002, 5, 381-382
  • A. Klöpffer, J. W. Engels, "Universal Fluorinated Nucleobases: Their Effect on the Catalytic Activity of Ribozymes" Coll. Symp. Series 2002, 5, 348-351
  • K. Jahn-Hofmann, J. W. Engels, "A New Concept for DNA-Arrays" Coll. Symp. Series 2002, 5, 335-337
  • S. Amberg, J. W. Engels, "Synthesis, Properties and Determination of the Configuration of Arylpropyl Backbone Modified Oligodeoxynucleotides" Coll. Symp. Series 2002, 5, 225-229
  • S. Amberg, J. W. Engels, "Synthesis and Properties of Nonpolar DNA (Arylalkyl)phosphonates"Helv. Chim. Acta 2002, 85, 2503-2517
  • J. Parsch, J. W. Engels, "C-F- - -H-C Hydrogen Bonds in Ribonucleic Acids" J. Am. Chem. Soc. 2002, 124 (20), 5664-5672
  • Engels, J.W.; Lehmann, T.J.; Parsch, J.; Strube, T. „Fluorine Substituted DNA and RNA“ Coll. Symp. Series 2002, 5, 245-250
  • P. Brodin, M. Pinskaya, M. Buckle, U. Parsch, E. Romanova, J. W. Engels, M. Gottikh, J.-F. Mouscadet "Disruption of HIV-1 Integrase-DNA Complexes by Short 6-Oxocytosine-Containing Oligonucleotides" Biochemistry 2002, 41, 1529-1538


  • Lehmann, T.J.; Serwe, M.; Caselmann, W.H.; Engels, J.W. „Design and Properties of Hepatitis C Virus Antisense Oligonucleotides for Liver Specific Drug Targeting” Nucleosides, Nucleotides 2001, 20, (4-7), 1343-1346
  • Brodin, P.; Pinskaya, M.; Parsch, U.; Bischerour, J.; Leh, H.; Romanova, E.; Engels, J.W.; Gottikh, M.; Mouscadet, J.F. “6-oxocytidine containing oligonucleotides inhibit the HIV-1 integrase in vitro” Nucleosides, Nucleotides 2001, 20, (4-7), 481-486
  • S. Amberg, J. W. Engels, "Phenylalkyl Backbone Modified Oligodeoxynucleotides, their Synthesis and the Influence of the Alkyl Chain" Nucleosides, Nucleotides & Nucleic Acids, 2001, 20, (4&7), 1275-1278
  • J. Parsch, J. W. Engels, "Stacking and Stability of RNA Duplexes Containing Fluorbenzene and Fluorobenzimidazole Nucleosides" Nucleosides, Nucleotides & Nucleic Acids, 2001, 20, (4&7), 815-818
  • T. Strube, O. Schiemann, F. MacMillan, T. Prisner, J. W. Engels, "A New Facile Method for Spin-Labeling of Oligonucleotides" Nucleosides, Nucleotides & Nucleic Acids,  2001, 20, (4&7), 1271-1274
  • C. Klebba, S. A. Klein, J. W. Engels, "An Assay System to Detect the Selective Advantage of Anti-HIV Ribozyme Expressing CD4 T-Lymphocytes" Nucleosides, Nucleotides & Nucleic Acids, 2001, 20, (4&7), 629-634
  • E. Duchart, C. Richter, B. Reif, S. Glaser, J. W. Engels, C. Griesinger, H. Schwalbe, "Measurement of 2J(H,C)- and 3J(H,C)-coupling constants by a/b selective HC(C)H-TOCSY" J. Biomol. NMR, 2001, 21, 117-126
  • T. Lehmann, J. W. Engels, "Synthesis and Properties of Bile Acid Phosphoramidites 5´-Tethered to Antisense Oligodeoxynucleotides Against HCV", Bioorg. & Med. Chem. 2001, 9, 1827-1835
  • G. Pappenberger, H. Aygün, J. W: Engels, U. reimer, G. Fischer, T. Kiefhaber, "Nonprolyl cis peptide bonds in unfolded proteins caus complex folding kinetics", Nature Struct. Biol. 2001, 8 (5), 452-458
  • Schwarzer, K.; Wojczewski, C.; Engels, J.W. “Synthesis of 3'-thioamido-modified 3'-deoxythymidine-5'-triphosphates and their use as chain terminators in Sanger-DNA sequencing” Nucleosides, Nucleotides 2001, 20(4-7), 879-82


  • Caselmann, W.H.; Serw, M.; Lehmann, T.; Ludwig, J.; Sproat, B. S.; Engels, J. W. “Design, delivery and efficacy testing of therapeutic nucleic acids used to inhibit hepatitis C virus gene expression in vitro and in vivo”  World Journal of Gastroenterology, 2000, 6(5), 626-629
  • Parsch, U.; Engels, J. W. “pH-Independent Triple-Helix Formation with 6-Oxocytidine as Cytidine Analogue“ Chemistry-A European Journal, 2000, 6(13), 2409-2424
  • Wojczewski, C.; Schwarzer, K.; Engels, J. W. “Synthesis of 3'-Thioamido-Modified 3'-Deoxythymidine 5'-Triphosphates by Regioselective Thionation and Their Use as Chain Terminators in DNA Sequencing” Helvetica Chimica Acta, 2000, 83(6), 1268-1277
  • J. W. Bats, J. Parsch and J. W. Engels, „1-deoxy-1-(4-fluorophenyl)-b-D-ribofuranose, is hemihydrate and 1-deoxy-1-(2,4-difluorophenyl)-b-D-ribofuranose: structural evidence for intermolecular C-H...F-C interactions“, Acta Cryst. 2000, C56, 201-205
  • C. Wojczewski, J. W. Engels „Synthesis of 3´-S-(2-Aminoethylthio)-3´-deoxythimidine-5´-triphosphates“ Synthesis, 2000, 149-153
  • J. W. Engels, E. Uhlmann „Chemistry of Oligonucleotides“ Pharmaceutical Aspects of Oligonucleotides, Patrick Couvreur and C. Malvy 2000, 35-68
  • J. W. Bats, U. Parsch and J. W. Engels „4.2´-Anhydro-5-(b-D-arabinofuranosyl)-uracil dihydrate and 4.2´-anhydro-5-(b-D-arabinofuranosyl)-uracil monohydrate. An example of solid state transformation“ Acta Cryst. 2000, C56, 1129-1131
  • C. Klebba, O. G. Ottmann, M. Scherr, M. Pape, J. W. Engels, M. Grez, D. Hoelzer, S. A. Klein „Retrovirally expressed anti-HIV ribozymes confer a selective survival advantage on CD4+ T cells in vitro“ Gene Therapy, 2000, 7, 408-416
  • T. Schoetzau, U. Koert, J. W. Engels „3´-Branched Thymidine 5´-Triphosphates: Synthesis, Application and Coupling with Near-Infrared Oxazine Fluorescent Dye“ Synthesis,  2000, 5, 707-713
  • Schoetzau, T.; Klingel, S.; Wartbichler, R.; Koert, U.; Engels, J.W. “Synthesis of a Fluorescent Derivative of 6-N-[N-(6-aminohexyl)carbamoyl)-2',3'-dideoxyadenosine 5'-triphosphate for Detection of Nucleic Acids” Perkin 1 2000, 9, 1411-1415


  • Schell, P.; Laux, W.; Engels, J.W. “Triarylmethyl Substituted 4,5-Dicyanoimidazoles as Activators for Rp-Diastereoselective Synthesis of TpN Dinucleoside Methylphosphonates” Nucleosides & Nucleotides, 1999, 18(6-7), 1169-1174
  • Scherr, M.; Maurer, A. B.; Klein, S.; Ganser, A.; Engels, J. W.; Grez, M. “Effective Reversal of a Transformed Phenotype by Retrovirus-mediated Transfer of a Ribozyme Directed Against Mutant N-ras. [Erratum to document cited in CA129:325814]“ Gene Therapy 1999, 6(1), 152
  • T. J. Lehmann, S. Eisenhardt and J. W. Engels, „Thermodynamic studies on hairpin forming antisense oligodeoxynuclotides directed against hepatitis C virus RNA“ Nucleosides & Nucleotides 1999, 18, 1689-1691
  • K. Stolze and U. Koert, and J. W. Engels „ Synthesis of 3´-sugar- and Base-Modified nucleotides and their application as potent chain terminators in DNA sequencing“ Helvetica Chimica Acta , 1999, 82, 1311-1323
  • U. Parsch, Karlheinz Wörner and J. W. Engels „Selective Recognition of triplex and duplex melting in triple-helical nucleic acids by circular dichroism spectroscopy“ Nucleosides & Nucleotides 1999, 18, 1641-1643
  • H. B. Lazrek, A. Rochdi, J. W. Engels „Synthesis of 5´-ethynyl-2´-deoxynucleoside analogues as building block for antisense oligonucleotides“ Nucleosides & Nucleotides 1999, 18, 1257-1259
  • C. Wojczewski, K. Stolze, J. W. Engels „Fluorescent Oligonucleotides - Versatile Tools as Probes and Primers for DNA and RNA Analysis“ Synlett, 1999, 10, 1667-1678
  • M. Alt, S. Eisenhardt, M. Serwe, R. Renz, J. W. Engels, W. H. Caselmann „Comparative inhibitory potential of differently modified antisense oligodeoxynucleotides on Hepatitis C virus translation“ European Journal of Clinical Investestigation, 1999, 29, 868-876
  • J. Parsch, J. W. Engels „C-F...H-C Hydrogen bonds in crystals of Fluorobenzene Ribonucleo-sides“ Collection Symposium Series 1999, 2, 11-14
  • U. Parsch, J. W. Engels „Calculation of thermodynamic data from UV melting curves of Triple Helical Nucleic Acids: A comparison of methods“ Collection Symposium Series 1999, 2, 15-21
  • T. Strube, J. W. Engels „ Synthesis and thermodynamic properties of different 2´-Fluoro modified UUCG Hairpin Loops“ Collection Symposium Series 1999, 2, 298-301
  • T. Lehmann, K. Schwarzer, M. Schweitzer, J. W. Engels „Thermodynamic and CD-spectroscopic characterization of self-complementary Methyl- and Benzyl-modified d(CGCpGCG) Hexamers“ Collection Symposium Series ,1999, 2, 302-305
  • K-H. Wörner, T. Strube, J. W. Engels „Synthesis and Stability of GNRA-Loop Analogs“ Helvetica Chimica Acta , 1999, 82, 2094-2104
  • M. Schweitzer, J. W. Engels „Sequence Specific Hybridization Properties of Methylphosphonate Oligodeoxynucleotides“ Jour. of Biomol. Str. & Dynamics, 1999, 16 (6), 1177-1185
  • Zscherp, C.; Aygun, H.; Ernd, C.; Engels, J. W.; Mantele, W. “Thermal Unfolding of Tendamistat Probed by Fourier-Transform Infrared Spectroscopy” Spectroscopy of Biological Molecules: New Directions, European Conference on the Spectroscopy of Biological Molecules, 8th 1999, 25-26


  • M. Schweitzer and J. W. Engels „ Methylphosphonate modified DNA Hairpin Loops“ Nucleotides & Nucleosides, 1998, 17 (1-3), 317-326
  • M. Scherr, A. Maurer, S. Klein, A. Ganser, J.W. Engels and M. Grez „Effective reversal of a transformed phenotype by retrovirus-mediated transfer of a ribozyme directed against mutant N-ras“ Gene Therapy, 1998, 5, 1227-1234
  • C. Richter, B. Reif, K-H. Wörner, S. Quant, J. P. Marino, J. W. Engels, C. Griesinger and H. Schwalbe „A new experiment for the measurement of nJ(C,P) coupling constants including 3J(C4´i, Pi) and 3J (C4´i, Pi + 1) in oligonuclotides“ J. Biomol. NMR, 1998, 12, 223-230
  • J. Balbach, S. Seip, H. Kessler, M. Scharf, N. Kashani-Poor and J. W. Engels „Structure and Dynamic Properties of the Single Disulfide-Deficient a-Amylase Inhibitor (C45A/C73A) Tendamistat: An NMR Study“ Proteins: Structure, Function and Genetics,  1998, 33, 285-294
  • J. W. Engels, M. Scherr, A. Ganser, M. Grez and V. Wittmann  „Modified Hammerhead Ribozymes as Potential Therapeutics“ Nucleosides & Nucleotides, 1998, 17, 1685-1696
  • H. B. Lazrek, J. W. Engels, W. Pfleiderer „Synthesis Of Novel Branched Nucleoside Dimers Cotaining A 1,2,3,-Triazolyl Linkage“ Nucleosides & Nucleotides, 1998, 17, 1851-1856
  • P. Schell, J. W. Engels, „Rp-Diastereoselective Synthesis of Dinucleoside Methylphosphonates by the Phosphoramidite Approach“ Tetrahedron Letters  1998, 39, 8629-8632


  • M. Mag, J. Muth, K. Jahn, A. Peyman, G. Kretschmar, J. W. Engels and E. Uhlmann „Synthesis and Binding Properties of Oligodeoxynucleotides Containing Phenylphosphon (othio) ate Linkages“ Bioorg. Med. Chem., 1997, 12, 2213-2220 
  • M. Scherr, M. Grez, A. Ganser and J. W. Engels „Specific Hammerhead Ribozyme-mediated Cleavage of Mutant N-ras mRNA in Vitro and ex Vivo“J. Biol.Chem. 1997 272, 14304-14313
  • K. Faulstich, K. Wörner, H. Brill and J. W. Engels „A Sequencing Method for RNA Oligonucleotides  Based on Mass Spectrometry“ Anal. Chem.  1997 69, 4349-4354
  • M. Scherr, C. Klebba, R. Häner, A. Ganser and J. W. Engels, „Synthesis and properties of hammerhead ribozymes stabilized against nucleases by different 2´-modifications: methoxyethoxy-, fluoro- and amino groups“ Bioorg.&Medicinal Chem. Lett . 1997 7,1791-1796
  • W. Kleinekofort, M. Schweitzer, J. W. Engels, B. Brutschy, „Analysis of double-stranded oligonucleotides by laser-inducted liquid beam mass spectrometry“Int. J. Mass.Spectrom. Ion Proc., 1997, 163, 1-4L
  • N. Schönbrunner, G. Pappenberger, M. Scharf, J. W. Engels, T. Kiefhaber „ Effect of Pre-formed Correct Tertiary Interactions on Rapid Two-State Tendamistat Folding: Evidence for Hairpins as Initiation Sites for ß-Sheet Formations“ Biochemistry , 1997, 36, 9057-9065
  • I. Seipelt, S. H. Hoffmann, J. Schmidt, J. W. Engels and T. Beckers, „Overexpression, Purification and Use of a Soluble Human Interleukin-4 Receptor a-chain/Iggl Fusion Protein for Ligand Binding Studies“ Biochem.and Biophys. Res. Commun ., 1997, 239, 534-542
  • B. Reif, V. Wittmann, H. Schwalbe and C. Griesinger and K-H. Wörner, K. Jahn-Hofmann and J. W. Engels and W. Bermel, „132. Structural Comparison of Oligoribonucleotides and Their 2´-Deoxy-2´-fluoro Analogs by Heteronuclear NMR Spectroscopy“ Helv.Chim.Acta , 80, 1997, 1952-1971
  • M. Schweitzer and J. W. Engels „Analysis of Oligonucleotides“ in Antisense-From Technology to Therapy Eds. R. Schlingensiepen ,W. Brysch, K.-H. Schlingensiepen 1997, 4, 78-103
  • K. Wörner, K. Faulstich and J. W. Engels „Recent Developments in Mass Spectrometry: RNA Sequencing“ Nucleosides & Nucleotides  1997, 16, 573-577
  • C. Wojczewski, K. Faulstich and J. W. Engels „Synthesis and application of 3´-amino-dye-terminators for DNA sequencing“ Nucleosides & Nucleotides, 1997, 16, 751-754
  • P. Schell and J. W. Engels „5-(2-(2-Methoxy) Bornyl) Tetrazole as Catalyst for Diastereo-selictive Synthesis of 2´-Deoxy Dinucleoside (3´, 5´)-Methylphosponates“ Nucleosides & Nucleotides , 1997, 16, 769-772
  • T. J. Lehmann, S. Eisenhardt and J. W. Engels „Synthesis and characterization of phosphate-modified DNA-dimers with fluorine as an uncharged ligand“Nucleosides & Nucleotides  1997, 16, 815-820


  • W. Samstag, S. Eisenhardt, W. B. Offensperger and J. W. Engels: "Synthesis and Properties of New Antisense Oligodeoxynucleotides containing Benzylphosphonate Linkages" Antisense & Nuc.Acid Drug Development  1996, 6, 153-156
  • S. H. Faß and J. W. Engels: „Influence of Specific Signal Peptide Mutations on the Expression and Secretion of the a-Amylase Inhibitor Tendamistat in Streptomyces lividans“ J.Biol.Chem , 1996, 271, 15244-15252
  • N. Schönbrunner, J. Wey, J. W. Engels, H. Georg and T. Kiefhaber „Native-like b-structure in a Trifluoroethanol-induced Partially Folded State of the All-b-sheet Protein Tendamistat“ J. Mol. Biol. 1996, 260, 432-445
  • M. Schweitzer, W. Samstag and J. W. Engels „Synthesis and Characteristics of a self-complementary Tetramer d(CGCG) with methylphosphonate linkages of defined stereochemistry“ Coll.Czech Chem.Commun. Special Issue  1996, 16, 213
  • T. Albrecht, R. Schwab, C. Peschel, J. W. Engels, T. Fischer, C. Huber, W. E. Aulitzky „Cationic lipide mediated transfer of c-abl and ber antisense oligonucleotides to immature normal myeloid cells: Uptake, biological effects and modulation of gene expression“ Ann. Hematol . 1996, 72, 73-79


  • M. A. Wolter, J. W. Engels, L. Montanarella, R. Tilio, S. Facchetti, „Influence of the Matrix on the Analysis of Small Oligoribonucleotides by Fast Atom Bombardment Mass Spectrometry", J. Mass. Spectrometry, 1995, 30, 485-491
  • E. Nordhoff, M. Karas, R. Cramer, S. Hahner, and F. Hillenkamp, A. Lezius, F. Kirpekar, K. Kristiansen, J. Muth, C. Meier and J. W. Engels: "Direct Mass Spectrometric Sequencing of Low-picomole Amounts of Oligodeoxynucleotides with up to 21 Bases by Matrix-assisted Laser Desorption/Ionization Masss Spectrometry", J.Mass Spectrometry  1995, 30, 99-112
  • H.-G. Schaible, J. W. Bats and J. W. Engels: "5´-Azido-2´-dideoxythymidine", Acta Cryst C. 1995, 24102412
  • M. Schweitzer, W. Samstag and J. W. Engels: "Phosphoramidites of (Rp) and (Sp) - Configurated Adenosine Methylphosphonate Dimers", Nucleosides & Nucleotides  14, 817 1995
  • K. Faulstich, S. Brandtner, R. Wechselberger, C. Griesinger and J. W. Engels: "A new class of nucleoside-peptide-dye conjugates: synthesis and application", Peptides, Verlag Escom Science Publ. Leiden, 1995 Editor H.L. S. Maia 753-754
  • J. W. Engels und M. Lötzbeyer „Protein Engineering in Enzyme Catalysis in Organic Synthesis“ Eds. K. Drauz und H. Waldmann VCH, Weinheim   1995, 929-943
  • T. Vogl, R. Brengelmann, H.J. Hinz, M. Scharf, M. Lötzbeyer and J. W. Engels: "Mechanism of Protein Stabilization by Disulfide Bridges: Calorimetric Unfolding Studies on Disulfide-deficient Mutants of the a-Amylase Inhibitor Tendamistat" J.Mol.Biol. 1995, 481-496
  • A. Muth and J. W. Engels "Force Field Calculations of RNA-Tetraloops" J.Mol.Model. 1995,1 97-98
  • R. G. Geissler. J. Muth, A. Mauerer, U. Mentzel, M. Mag, J. W. Engels, D. Hoelzer, A. Ganser „ Modified antisense oligodeoxynucleotides against the splice acceptor site of tat do not inhibit in vitro hematopoietic colony growth in HIV-positive patients“ Ann. Hematol . 1995, 72, 89-95
  • M. A. Wolter and J. W. Engels „Nanoelectrospray mass spectrometry for the analysis of modified oligoribonucleotides“ Europ. Mass  Spec. 1995, 1, 583-590
  • J. F. Connell, M. Scharf, J. W. Engels, K. Withrich, "The Nuclear Magnetic Resonance 
         Solution Structure of the Mutant a-Amylase Inhibitor Tendamistat (R19L) and Comparison 
         with wild type Tendamistat", Eur. J. of Biochemistry, 1994, 220, 763-770


  • M. K. Herrlein, R. E. Konrad, J. W. Engels, T. Holletz, D. Cech, "Synthesis and Application of 3'-Amino modified Nucleotides and Oligodeoxynucleotides", Helv. Chim. Acta, 1994, 77, 586-596
  • M. Mag, J. W. Engels, "Synthesis of Dinucleotides Containing a Bridged Non-Chiral Internucleotide 5´- or 3´-Phosphoramidate Linkage", Tetrahedron, 1994, 34, 10225-10234
  • A. Frauendorf, J. W. Engels, "Interaction of linear and folded modified antisense Oligonucleotides with sequences containing secondary structure elements", Bioorg. Med. Chem. Lett., 1994, 4, 1019-1024
  • I. Demirhan, O. Hasselmayer, D. Hofmann, A. Chandra, F. P. Svinaruchk, V. V. Vlassov, J. W. Engels, P. Chandra, "Gene-Targeted Inhibition of Transactivation of Human immunodeficiency Virus Type1(Hiv-1)-LTR By Antisense Oligonucleotides", Virus Genes 9, 113-119, 1994
  • P. Rosmanitz, S. Eisenhardt , J. W. Bats, J. W. Engels, "New Proline derived Chiral Building Blocks for Nucleoside Methylphosphonate Synthesis", Tetrahedron, 1994, 19, 5719-5734
  • I. Seipelt, J. W. Engels, "Isolation and refolding of mutant des-methionine IL-2 Receptor a-chain synthesized as a fusion protein in Escherichia coli", Eur. J. Biochem. 1994, 223, 285-292
  • E. V. Vyazovkina, E. V. Savchenko, S. G. Lokhov, J. W. Engels, E. Wickstrom, "Synthesis of specific diastereomers of a DNA methylphosphonate heptamer, d(CpCpApApApCpA) and stability of base pairing with the normal DNA octamer d(TpGpTpTpTpGpGpC)", Nucleic Acids Res., 1994, 22, 2404-2409
  • S. Quant, R. W. Wechselberger, M. A. Wolter, K-H. Wörner, P. Schell J. W. Engels, C. Griesinger, H. Schwalbe, "Chemical Synthesis of 13C-labelled Monomers for the Solid-Phase and Template Controlled Enzymatic Synthesis of DNA- and RNA-Oligomers", Tetrahedron Lett., 1994, 35, 6649-6652
  • K. Faulstich, M. Herrlein, S. Brandtner, R. Konrad, C. Griesinger, J. W. Engels, "A new class of 3´-modified nucleotides as chain-terminators for DNA-sequencing“ Bioorg. Med. Chem. Lett., 1994, 4, 1975-1978


  • A. Frauendorf, J. W. Engels, "Automated Synthesis, Structure and Biological Activity of Backbone-modified Oligonucleotides", Studies in Natural Products Chemistry, Elsevier 1993, Ed. Atta-ur-Rahman, 13, 257-294, Elsevier
  • J. W. Engels, B. Sprunkel, E. Uhlmann, "DNA Synthesis", Biotechnology Second Edition 1993, 2, 317-369, Eds. H.J. Rehm, G. Reed, A. Pühler and P. Stadler, VCH Weinheim
  • E. V. Vyazovkina, A. V. Lebedev, J. W. Engels, "Determination of the absolute configuration for the diastereomers of all 16 Deoxydinucleoside methylphosphonates by 2-D NMR ROESY", Tetrahedron, 1993, 49, 1043-1052
  • E. V. Vyazovkina, J. W. Engels and A. V. Lebedev „Investigation of Diastereomers of non-ionic oligonucleotid analogs VIII. Assignment of absolute configurations of diastereomeric  deoxydinucleoside methylphosphonates by 2D NMR ROESY spectroscopy Bioorg. Chem. (Russia) 1993, 19, 197-210
  • S. Haas-Lauterbach, M. Scharf, B. Sprunkel, J. W. Engels, "High yield Fermentation and Purification of Tendamistat Analogues secreted by Streptomyces lividans", Appl. Microbiol. Biotechnol. 1993, 38, 719-727
  • H. Schwalbe, W. Samstag, J. W. Engels, W. Bermel, C. Griesinger, "Determination of 3J(C,P) and 3J(H,P) Coupling Constants in Nucleotide Oligomers", J. Biomolecular NMR, 1993, 479-486


  • J. W. Engels and K. Koller, "Gene Expression and Secretion of Eukaryotic Foreign Proteins  in Streptomycetes, in "Transgenesis-Applications of Gene Transfer", John Wiley &Sons 1992, 2, 32-53
  • T. Schmitt-John, J. W. Engels, "Promoter constructions for an efficient secretion-expression in Streptomyces lividans", Appl. Microbiol. Biotechnol. 1992, 36, 493-498
  • M. Renner, H-J. Hinz, M. Scharf, J. W. Engels, "Thermodynamics of Unfolding of the a-Amylase Inhibitor Tendamistat: Correlations between accessible surface area and heat capacity", J.Mol.Biol. 1992, 223, 769-779
  • T. Daum, J. W. Engels, M. Mag, J. Muth, S. Lüking, H. C. Schröder, E. Matthes, W.E.G. Müller, "Antisense Oligodeoxynucleotide: Inhibitor of Splicing of mRNA of Human Immuno-deficiency Virus, Intervirology, 1992, 33, 65-75
  • W. Samstag, J. W. Engels, "Stereoselektive Synthese phosphatmodifizierter DNA-Bausteine", Angew. Chem., 1992, 104, 1367-1369
  • M. Mag, R. Schmidt, J. W. Engels, "Synthesis and Selective Cleavage of an Oligodeoxynucleotide Containing a Bridged Non-Chiral Internucleotide 3'-Phosphor-amidate Linkage", Tetrahedron Lett., 1992, 33, 7319-7322
  • C. Meier, J.W. Engels, "Peptide Nucleic Acids (PNAs)- Unusual Properties of Nonionic Oligonucleotide Analogues", Angew. Chem., Int.Ed. Engl., 1992, 31, 1008-1010


  • J. W. Engels, T. Löschner, A. Frauendorf, "Diastereoselective synthesis of thymidine-methylphosphonate dimers", Nucleosides and Nucleotides, 1991, 10 (1-3), 347-350
  • M. Mag, J. Muth, S. Lüking, L. Biesert, and J. W. Engels, "HIV inhibiton by antisense oligodeoxynucleotides", Nucleosides and Nucleotides, 1991, 10 (1-3), 627-629
  • M. Mag, S. Lüking, and J. W. Engels, "Synthesis and selective cleavage of an oligodeoxy­nucleotide containing a bridged internucleotide 5'-phosphorothioate linkage", Nucleic Acids Res. 1991, 19, 1437
  • J. W. Engels, T. Daum, A. Frauendorf, S. Lücking, M. Mag, W.E.G. Müller, J. Muth, P. Rosmanitz, "Backbone Modified Oligonukleotides, Stereoselective Synthesis of Methylphosphonates and HIV Inhibitory Capacity of Phosphorothioates", Nucl. Acids. Res., Symposium 1991, Series No. 24, 83-86


  • T. Beckers, H. Hauser, D. Hüsken, J. W. Engels, "Analysis of soluble des-methionine mutant Interleukin-2 Receptor a chain (Tac protein) produced by transfected mamma­lian cells", Eur. J. Biochem. 1990, 189, 657-665
  • E. Bender, K. Koller, and J. W. Engels, "Secretory expression of human Interleukin-2 by Streptomyces lividans", Gene 1990, 86, 227-232
  • D. Hüsken, T. Beckers, J. W. Engels, "Overexpression in Escherichia coli of a methio­nine-free designed interleukin-2 receptor (Tac protein) based on a chemically cleavable fusion protein", Eur. J. Biochem. 1990, 193, 387-394
  • E. Bender, R. Vogel, K. Koller und J. W. Engels, "Synthesis and secretion of hirudin by Streptomyces lividans", Appl. Microbiol. Biotechnol. 1990, 34, 203-207
  • J. Glauder, H. Ragg, J. Rauch, and J. W. Engels, "Human-Peptidylglycine a-amidating Monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells", Biochem. Biophy. Res. Commun. 1990, 169 (2), 551-558
  • T. Löschner and J. W. Engels, "Diastereomeric Dinucleoside-Methylphosphonates: Determination of configuration with the 2-D NMR ROESY technique", Nucl. Acids Res. 1990, 18, 5083-5088
  • J. W. Engels und S. Haas-Lauterbach, "Protein Design am Beispiel des Tendamistats", GIT Fachz. Lab. 1990, 9, 1037-1044.
  • J. W. Engels and T. Löschner, "Synthetic Antisense Oligonucleotides", J. Clin. Chem. Clin. Biochem. 1990, 28 (5)


  • J. W. Engels, E. Uhlmann, "Gensynthese", Angew. Chem. 1989, 101, 733, Int.Ed.Engl. 1989, 28, 716.
  • M. Scharf, J. W. Engels, D. Tripier, "Primary structures of new Iso-hirudins", FEBS Letters 1989, 255, 105
  • M. Mag, J. W. Engels, "Synthesis and selective cleavage of oligodeoxyribonucleotides con­taining non chiral internucleotide Phosphoramidate linkages", Nucleic Acids Res. 1989, 17, 5973
  • T. Löschner, J. W. Engels, "One Pot RP-Diastereoselective Synthesis of Dinucleoside Methylphosphonates using Methyldichlorophosphine", Tetrahedron Lett. 1989, 30, 5587


  • J. W. Engels, E. Uhlmann, "Gene Synthesis", Adv. in Biochemical Engineering/Biotechnology, 1988, 37, 74, Springer Verlag
  • T. Beckers, H. J. Hauser, D Hüsken, J. W. Engels, "Expression and characterization of a des-Met mutant Interleukin-2 receptor (Tac-protein) with Interleukin-2 binding affinity" Journal of Biological Chemistry, 1988,  263, No. 17, 8359
  • M. Mag, J. Engels, "Synthesis and structure assignments of amide protected nucleosides and their use as phosphoramidites in deoxyoligonucleotide synthesis", Nucleic Acids Res. 1988, 16, 3525
  • M. Mag, J. W. Engels, "Phosphoramidite analogs of dinucleotides: Synthesis and 1H assignment by two dimensional NMR spectroscopy (1H,1H-COSY)", Nucleosides and Nucleotides  1988, 7, 725
  • T. Löschner, J. W. Engels, "Methylphosphonamidites: Preparation and application in oligonucleoside methylphosphonate synthesis", Nucleosides and Nucleotides 1988, 7, 729


  • M. Korth, J. Engels, M. Schäfer-Korting, "Influcence of phosphodiesterase inhibition and of carbachol on inotropic effects of 8-substituted cyclic AMP analogues", Naunyn-Schmiedeberg's Arch. Pharmacol. 1987, 335, 166
  • J. W. Engels, W. Becker, T. Beckers, D. Hüsken W. Wetekam, "Chemoenzymatic Synthesis of a gene for the interleukin-2 receptor", Proceedings of 15th IUPAC Int. Symposium on the Chemistry of Natural Products,Pure & Appl. Chem., 1987, 59 ,  437-444
  • J. W. Engels, J. Glauder, H. Müllner, E. Uhlmann, W. Wetekam, T. Hashimoto Gotoh, B. Scheikl-Lenz, "Chemoenzymatic gene synthesis of a gene for human growth hormone releasing hormone (hGHRH), its expression on E. coli and enzymatic amidation", Nucleosides and Nucleotides 1987, 6 (1+2), 185-195
  • J. W. Engels, J. Glauder, H. Müllner, D. Tripier, E. Uhlmann, W. Wetekam, "Enzymatic amidation of recombinant (Leu27) growth hormone releasing hormone-gly45Protein", Engineering 1987, 1, (No.3), 195-199
  • J. W. Engels, A Glossary "Gene Technology" auf Anregung von IUPAC, Fachgruppe Medicinal Chemistry, Blackwell Scientific, Oxford 1987.
  • J. W. Engels, P. Artelt, T. Beckers, H.-J. Hauser, D. Hüsken, "Expression of a Synthetic Gene for Interleukin-2 Receptor in E. coli and Mammalian Cells", Biological Chemistry, Hoppe-Seyler, 1987, 368 (Nr. 9), 1038
  • J. W. Engels, E. Bender, K. P. Koller, "Secretory expression of interleukin-2 by Streptomyces lividans", Biological Chemistry, Hoppe-Seyler, 1987, 368 (Nr.9), 1038
  • J. W. Engels, E. Bender, M. Neeb, K. P. Koller, H. Wallmeier, "Site Specific Mutagenesis on the a-Amylase Inhibitor Tendamistat", Biological Chemistry, Hoppe-Seyler, 1987, 368 (Nr.9), 1039
  • M. Korth, J. W. Engels, "Inotropic and electrophysiological effects of 8-substituted cyclic AMP analogues on guinea-pig papillary muscle", Naunyn-Schmiedeberg's Arch. Pharmacol. 1987, 335


  • J. W. Engels, E. Uhlmann, Chemical 5'-Phosphorylation of Oligonucleotides valuable in automated DNA synthesis", Tetrahedron Letters 1986, 27 (Nr. 9), 1023
  • E. Uhlmann, J. W. Engels, "Automated 5'-Phosphorylation of Oligodeoxyribonucleotides", Chemica Scripta 1986, 26, 217-219
  • U. Eisel, W. Jarausch, K. Goretzki, A. Henschen, J. W. Engels, U. Weller, M. Hudel, E. Habermann, H. Niemann, "Tetanus toxin: primary structure, expression in E. coli and homology with botulinum toxins", The EMBO Journal 1986, 5 (No. 10), 2495-2502


  • J. W. Engels, M. Leineweber, E. Uhlmann, "Chemoenzymatic Synthesis of a Gene Control Region", Nucleosides and Nucleotides 1985, 4, 183
  • E. Uhlmann, J. W. Engels, W. Wetekam, "Signal Sequence: Application of Chemical DNA-Synthesis to Studies of Translocation of Expressed Protein, Nucleosides and Nucleotides 1985, 4, 259
  • J. W. Engels, "Chemie der Gene", ƒrzteblatt Rheinland-Pfalz, Nov. 1985, 11, 38. Jahrg., 609-612,


  • J. W. Engels, A. Jäger, "Synthesis of Deoxynucleoside methylphosphonates via a phosphonamidite approach", Tetrahedron Letters 1984, 25, 1437
  • J. W. Engels, M. Leineweber E. Uhlmann, Chemoenzymatische Synthese eines funktionellen Gens für Human Gamma Interferon", Angew. Chem. 1984, 96, , 969
  • K.-P. Koller, J. Engels, E. Uhlmann, "Gene Amplification and Overproduction of the a-Amylase Inhibitor in Streptomyces Tendae", Third European Congress on Biotechnology, 1984, 3, 273, Verlag Chemie, Weinheim.
  • J. Engels, M. Leineweber, E. Uhlmann, "Chemical and Enzymatic Synthesis of Genes", Proceedings of the Vth International Conference on Organic Synthesis, Freiburg, 27.-30. August 1984


  • J. Nargeot, J. M. Narbonne, J. Engels, H. Lester, "Time course of the increase in myocardial slow inward current after a photochemically generated concentration jump of intracellular cyclic AMP", Proc. Natl. Acad. Sci. USA 1983, 80, 2395
  • O. K. Sharma, J. Engels, A. Jäger, R. Crea, J. van Boom, B. B. Goswami, "3'-O-Methylated Analogs of 2-5A as inhibitors of virus replication", FEBS Letters 1983, 158, 298


  • M. Korth, J. Engels, "The positive inotropic effect of cAMP derivatives and their interaction with cAMP",Naunyn-Schmiedeberg's Arch. Pharmacol. 1982, 317
  • J. Engels, A. Jäger, "Synthese und Eigenschaften von 8-substituierten Adenosin-3',5'-phosphorsäure-benzylestern", Archiv der Pharmazie 1982, 315, 368
  • J. Engels, U. Krahmer, L. Zsolnai, G. Huttner, "8-Chinolinsulfonsäure-(3-nitro-1,2,4-triazolid), ein neues, effektives Kondensationsmittel für die Oligonukleotid-Synthese" Liebigs Ann. Chem. 1982, 745
  • J. Engels, "DNA Synthesis by the Phosphite Triester Approach", Leuven 1982, EMBO Course Manual 1982, 120
  • J. Engels, A. Jäger, "Ein neuer Weg zur Synthese von Nucleosidmethylphosphonaten", Angew. Chem. 1982, 94, 931


  • J. Engels, "Synthesis of 2'-end modified 2',5'-Adenylate Trimers", Tetrahedron Letters 1981, 4339
  • J. Engels, U. Krahmer, "Bis-(2,2,2-tribromoethyl)-phosphochloridate: A suitable Phosphorylating Agent for Nucleosides", Synthesis 1981, 6, 485
  • A. Jäger, J. Engels, "Synthesis of Methylphosphonate and Methylphosphorotriester Analogues of 2',5'-Adenylate Trimers", Nucl. Acids Res. Symp. 1981, Series 9, 149
  • J. Engels, A. Jäger, M. Korth, "Effects of 8-substitued Cyclic AMP Benzyl Esters on Guinea Pig Ventricular Myocardium", Adv. Cyclic Nucleotide Res. 1981, 14, 689
  • "Analysis of the positive inotropic effect of C-8-substituted cAMP Benzyl Esters on Guinea Pig Papillary Muscle", Naunyn-Schmiedeberg's Arch. Pharmacol. 1981, 316 (R34)


  • J. Engels, "Zur Elektrochemischen Reduktion von Diphenylphosphorsäure-?-halogen-ethylestern", Liebigs Ann. Chem. 1980, 557 (1980).
  • J. Engels, "Synthesis and Reactivity of Guanosine-3',5'-cyclophosphoric Acid Alkylesters", Bioorganic Chem. 1979, 8, 9
  • M. Korth, J. Engels, "The Effects of Adenosine and Guanosine-3',5'-phosphoric Acid Benzyl Esters on Guinea Pig Myocardium", Naunyn-Schmiedeberg's Arch. Pharmacol. 1979, 310, 103
  • J. Engels, "Zur Schutzgruppenproblematik in der Nukleotid-Chemie", Habilitationsschrift, Konstanz 1979
  • J. Engels, "Selektive elektrochemische Schutzgruppenabspaltung in der Nukleotid-Synthese", Angew. Chem. 1979, 91, 155
  • J. Engels U. Krahmer, "Gezielte Synthese des trimeren Isoadenylats A2'p5'A2'p5'A", Angew. Chem. 1979, 91, 1007
  • M. Korth J. Engels, "Adenosine-3',5'-phosphoric Acid Benzyl Esters as possible Transport Forms of cAMP across the Cell Membrane", Naunyn-Schmiedeberg's Arch. Pharmacol. 1979, 308 (R32), 127
  • J. Engels, R. Reidys, "Synthesis and Application of the Photolabile Guanosine-3',5'-phosphoric-o-nitrobenzylester", Experientia 1978, 34, 14
  • J. Engels, "On the Electrochemical Removal of Several 2-Haloethyl-Moieties and its Application to Nucleotide Chemistry", Nucl. Acids Res. Spec. Publ. 1978, 4, 31
  • J. Engels, E. J. Schlaeger, "Synthesis, Structure and Reactivity of Adenosine Cyclic 3',5'-phosphate Benzyl Triesters", J. Med. Chem. 1977, 20, 907
  • J. Engels, J. Hoftiezer: "Synthese und Eigenschaften von Uridin-3',5'-cyclophosphat-estern", Chem. Ber. 1977, 110, 2019
  • W. Depmeier, J. Engels, K.-H. Klaska, "The Crystal and Molecular Structure of 2'-Acetyluridine-3',5'-cyclophosphate Benzyl Triester, a Pyrimidine Nucleotide with Syn-Conformation", Acta Crystallogr. 1977, 33, 2436
  • J. W. Engels, "Zum Mechanismus der Hydrolyse des Adenosin-3',5'-cyclo-phosphatbenzylesters", Z. Naturforsch. 1977, 32b, 807
  • J. Engels W. Pfleiderer: "Synthese und Eigenschaften von Uridin-3',5'-cyclophosphat-triestern", Tetrahedron Letters, 1975, 1661
  • J. Engels, W. Pfleiderer, "Synthesis and Reactivity of Nucleoside Cyclophosphate Triesters", Nucl. Acids Res. Spec. Publ. 1975, 1, 113
  • J. Engels, "Ein Beitrag zur Reduktion aromatischer Nitro- und Nitroso-Verbindungen mit Verbindungen des dreiwertigen Phosphors", Dissertation Regensburg 1972
  • J. Sauer J. Engels: "Ein Beitrag zur Reduktion aromatischer Nitro- und Nitrosoverbindungen mit dreiwertigen Phosphorverbindungen", Tetrahedron Letters, 1969, 5175
  • K.-J. Range, G. Engert, J. Engels, "Über Hochdruckphasen des AgInTe2 und CuInTe2 mit NaCl-Struktur", Z. Naturforsch. 1968, 23, 1008
  • K.-J. Range, J. Engels, A. Weiß, "Über Hochdruckphasen des CuInSe2 und AgInSe2 mit dichter Zinkblende-Struktur", Z. Naturforsch. 1968, 23, 1262


  • J. W. Engels, "Substituierte Adenosin-3',5'-phosphorsäurebenzyltriester, Verfahren zu ihrer Herstellung und diese Verbindungen enthaltende Arzneimittel", DE 30272 79.
  • J.W. Engels, W. König, H. Müllner, E. Uhlmann und W. Wetekam, "Herstellung vom Polypeptiden mit einem Säureamid-Carboxyterminus", DE 33270 07.
  • W. König, J. W. Engels, E. Uhlmann und W. Wetekam, "Herstellung von Sekretin", DE 33287 93.
  • J. Engels und A. Jäger, "Verfahren zur Herstellung von Nukleosidalkyl-, -aralkyl- und -arylphosphoniten und -phosphonaten", DE 33320 68.
  • J. W. Engels, M. Leineweber, E. Uhlmann und W. Ulmer, "Gentechnologisches Verfahren zur Herstellung von Human-Gamma-interferon und Mittel zur Durchführung dieses Verfahrens", DE 34099 66.
  • J. W. Engels, M. Leineweber, E. Uhlmann und W. Ulmer, "Herstellung von Polypeptiden mit Human-Gammainterferon-Aktivität", DE 34148 31.
  • J. W. Engels, E. Uhlmann, F. Wengenmayer, H. Müllner, E.-L. Winnacker, R. Mertz und H. Okazaki, "Gentechnologisches Verfahren zur Herstellung von Human-Interleukin-2 und Mittel zur Durchführung dieses Verfahrens", DE 34199 95.
  • D. Brauer, J. W. Engels, P. Habermann, E. Uhlmann und F. Wengenmayer, "Gentechnologisches Verfahren zur Herstellung von Hirudinen und Mittel zur Durch­führung dieses Verfahrens", DE 34294 30.
  • J. W. Engels, E. Uhlmann und M. Leineweber, "Synthetische Regulationsregion", DE 34306 83.
  • J. W. Engels, M. Leineweber, E. Uhlmann und W. Wetekam, "Synthetische Signalsequenz zum Transport von Proteinen in Expressionssystemen", DE 34268 18.
  • J. W. Engels, E. Uhlmann, W. Wetekam, W. König und H. Müllner, "Arzneimittel mit GRF-Wirkung", DE 34368 19.
  • J. W. Engels, "Verfahren zur Herstellung von Corticosteroid-2l-phosphorsäuren und deren Salzen und die Corticosteroid-2l-phosphors‰uretriester", DE 34407 94.
  • J. W. Engels und E. Uhlmann, "Verfahren und Mittel zur Phosphorylierung", DE 35078 81.
  • J. W. Engels, M. Leineweber, E. Uhlmann und F. Wengenmayer, "Veränderung der DNA-Sequenz zwischen Shine-Dalgarno-Sequenz und Startcodon des trp-Operons zur Steigerung der Proteinexpression", DE 35141 13.
  • J. W. Engels, M. Leineweber, G. Seipke, E. Uhlmann, W. Ulmer und W. Wetekam, "Biologisch aktive Derivate des Human- g-Interferons, ihre Herstellung und Arzneimittel, die solche Derivate enthalten", DE 35369 39.
  • J. W. Engels, E. Uhlmann, F. Wengenmayer, H. Müllner, M. Leineweber, E.-L. Winnacker und R. Mertz", Derivat des Interleukin-2, seine Herstellung und Verwendung", DE 35374 61.
  • K.-P. Koller, J.W. Engels, M. Neeb, "Tendamistat-Derivate", DE 3707150 A1.
  • D. Tripier, P. Crause, P. Habermann, M. Kramer, M. Scharf, J.W. Engels, "Neue Iso­hirudine", DE 3835815.8j
  • J. W. Engels, M. Herrlein und R. Konrad, "3'(2')- Amino und thiol modifizierte Nukleoside und Nukleotide",DE 08184759
  • J. W. Engels, M. Herrlein, R. Konrad, M. Mag 3´-(2´)- Amino or thiol-modified, Fluorescent Dye-coupled oligonucleotides and a process for the preparation and the use Thereof, United States Patent No. DE 08184759
  • K. Faulstich, S. Brandtner, R. Wechselberger, J. W. Engels, C. Griesinger, „´3-(4´-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer“ DE 4418691.6
  • E. Uhlmann, J. W. Engels, M. Scherr, A. Ganser Reagent and method for inhibition of N-ras expression HOE96 F 138
  • K. H. Wörner, K. Faulstich, H. Brill, J .W. Engels „Ein neues Verfahren zur Sequenzierung von Biopolymeren mit Massenspektrometrie“ 197 14 558.2 eingereicht
    ·   K. Faulstich, C. Wojczewski, J. W. Engels „ DNA-Sequenzierung mittels 
    Post-Sequencing-Labelling (PSL)“ Deutsche Patentanmeldung Nr. 199 55 555.9