Picture of the month: August 2021

Iotm aug 2021 big

Multicolor imaging with exchangeable fluorophore labels

Fluorophore labels that transiently bind their target (e.g. PAINT-labels in SMLM and STED, or "exchangeable" labels) are a powerful tool to bypass photobleaching in microscopy. They enable a time efficient experimental protocol to visualize different cellular structures in cells with a high labeling density. This image shows multiple cellular structures of a single cell visualized using exchangeable fluorophore labels and a confocal laser scanning microscope. The endoplasmic reticulum (green), actin (blue), mitochondria (red), DNA (orange) and cellular membranes (magenta) are shown.

Picture of the month: April 2021

Iotm apr 2021

DNA-PAINT imaging of the endoplasmic reticulum

The ER-membrane protein FAM134B was imaged in U2OS cells using DNA-PAINT. The membrane protein was labeled in chemically fixed cells with a primary antibody targeting the membrane protein and short DNA docking strand labeled secondary antibodies. Using complementary fluorescently labeled DNA strands (R2-ATTO655) and HILO mode for sequential image acquisition allowed reconstruction of the endoplasmic reticulum network over the whole cell area (i) and reveals fine structural details of the endomembrane system (ii, magnified region shown in (i)). Scale bar is 10 µm (i) and 1 µm (ii), spatial resolution ~ 40 nm (determined through decorrelation analysis, Descloux et al., Nat Meth 2019).

Picture of the month: December 2020

Iotm dec 2020

Synaptic cleft

A four-color super-resolution image of brain tissue.

Picture of the month: August 2020

Iotm aug 2020

PAINT imaging of E. coli

Confocal laser scanning microscopy (CLSM) enables to study cellular structures with diffraction‑limited resolution. With the help of super-resolution microscopy the membrane and the defined filigree structure of the nucleoid can be visualized with near-molecular spatial resolution even in small organisms like E. coli. (scale bar 1 μm)

Picture of the month: January 2020

Iotm jan 2020

Recent article in Science Signaling

Single-molecule imaging reveals the oligomeric state of functional TNFα-induced plasma membrane TNFR1 clusters in cells.

Picture of the month: September 2019

Iotm sep 2019

Recent Cover in Neurophotonics

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Picture of the month: April 2019

Iotm apr 2019

Exchange-DNA-PAINT of the receptor tyrosine kinases EGFR and MET

The receptor tyrosine kinases epidermal growth factor receptor (EGFR, magenta) and mesenchymal epithelial transition factor receptor (MET, cyan) were imaged in unstimulated HeLa cells using Exchange-DNA-PAINT. Receptors were modified with short DNA docking strands (P1 and P5) via immunostaining and imaged sequentially after addition of the corresponding imager strands labeled with ATTO 655. These multi-color images facilitate co-localization and interaction analysis of receptors on the cell membrane. Scale bar 5 µm, insets 5 x 5 µm.

Picture of the month: August 2018

Iotm aug 2018

Fixation matters

After infection, Salmonella establish a tubular network in the host cell. This network is very sensitive to osmotic changes and is destroyed upon formaldehyde fixation, leading to the formation of artificial vesicular structures (left image). Glutaraldehyde fixation preserves the network and reveals Salmonella-induced filaments with ~ 150 nm diameter (right image). Dual-color dSTORM measurements of the secreted Salmonella effector protein SseJ (Alexa Fluor 647, red hot) and the host cell lysosomal marker LAMP-1 (Alexa Fluor 532, green). Salmonella nucleoids are counterstained with DAPI (blue). Scale bars are 1 µm.

Picture of the month: March 2018

Conf sted 01

STED image of microtubuli

Comparison of confocal vs STED pictures of our new homebuilt STED setup. Microtubuli stained with Abberior Star635.

Picture of the month: November 2017

Iotm nov 2017

Recent cover in Science Signaling

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Picture of the month: June 2017

Iotm june 2017

Recent cover in nature microbiology

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(Image: Ella Maru Studio, Cover Design: Karen Moore)

Picture of the month: September 2016

Iotm sep 2016

Quantitative analysis of SMLM data with LAMA

A list of single-molecule localizations serves as input data (top) for LAMA and is post-processed for clustering (Ripley functions, DBSCAN, OPTICS), single-molecule counting, colocalization analysis and localization precision determination.

Picture of the month: June 2016

Iotm jun 2016

PAINT microscopy image of E.coli

Membrane stained with Nile Red (red) and chromosomal DNA with JF-646-Hoechst (blue).

Picture of the month: January 2016

Iotm jan 2016

Confocal fluorescence image of a dividing cell

Stained for DNA (blue), actin (red) and tubulin (green).

Picture of the month: October 2015

Iotm oct 2015

Single molecule FRET on DNA

Picture of the month: September 2015

Iotm sep 2015 lowres

Imaging neurons

Fine structure of a neuron's membrane recorded with super-resolution imaging (CB).

Picture of the month: August 2015

Iotm aug 2015 lowres

dSTORM imaging of microtubules

Image of an HeLa cell stained for microtubules.