Krueger Lab

2021

Enhanced differentiation of functional human T cells in NSGW41 mice with tissue-specific expression of human interleukin-7.
Coppin E, Sundarasetty BS, Rahmig S, Blume J, Verheyden NA, Bahlmann F, Ravens S, Schubert U, Schmid J, Ludwig S, Geissler K, Guntinas-Lichius O, von Kaisenberg C, Groten T, Platz A, Naumann R, Ludwig B, Prinz I, Waskow C, Krueger A.
Leukemia. 2021 May 11.

Modeling the Dynamics of T-Cell Development in the Thymus.
Robert PA, Kunze-Schumacher H, Greiff V, Krueger A.
Entropy (Basel). 2021 Apr 8;23(4):437.


2020

The transcription factor TAL1 and miR-17-92 create a regulatory loop in hematopoiesis.
Meyer A, Herkt S, Kunze-Schumacher H, Kohrs N, Ringleb J, Schneider L, Kuvardina ON, Oellerich T, Häupl B, Krueger A, Seifried E, Bonig H, Lausen J.
Sci Rep. 2020 Dec 8;10(1):21438.

The Role of MicroRNAs in Development and Function of Regulatory T Cells - Lessons for a Better Understanding of MicroRNA Biology.
Kunze-Schumacher H, Krueger A.
Front Immunol. 2020 Sep 9;11:2185.

MicroRNA miR-181-A Rheostat for TCR Signaling in Thymic Selection and Peripheral T-Cell Function.
Grewers Z, Krueger A.
Int J Mol Sci. 2020 Aug 27;21(17):6200.

Enhanced differentiation of functional human T cells in NSGW41 mice with tissue-specific expression of human interleukin-7
Emilie Coppin, Bala Sai Sundarasetty, Susann Rahmig, Jonas Blume, Nikita A. Verheyden, Franz Bahlmann, Sarina Ravens, Undine Schubert, Janine Schmid, Stefan Ludwig, Constantin von Kaisenberg, Alexander Platz, Ronald Naumann, Barbara Ludwig, Immo Prinz, Claudia Waskow, Andreas Krueger
bioRxiv 2020.04.24.060319.

Vascular miR-181b controls tissue factor-dependent thrombogenicity and inflammation in type 2 diabetes.
Witkowski M, Witkowski M, Saffarzadeh M, Friebel J, Tabaraie T, Ta Bao L, Chakraborty A, Dörner A, Stratmann B, Tschoepe D, Winter SJ, Krueger A, Ruf W, Landmesser U, Rauch U.
Cardiovasc Diabetol. 2020 Feb 17;19(1):20.

MicroRNA-181a regulates IFN-γ expression in effector CD8+ T cell differentiation.
Amado T, Amorim A, Enguita FJ, Romero PV, Inácio D, de Miranda MP, Winter SJ, Simas JP, Krueger A, Schmolka N, Silva-Santos B, Gomes AQ.
J Mol Med (Berl). 2020 Feb;98(2):309-320.

Magnetic Bead-Based Enrichment of Murine MAIT Cells.
Winter SJ, Krueger A.
Methods Mol Biol. 2020;2098:299-305.


2019

MicroRNA miR-181a/b-1 controls MAIT cell development.
Winter SJ, Kunze-Schumacher H, Imelmann E, Grewers Z, Osthues T, Krueger A.
Immunol Cell Biol. 2019; 97(2):190-202.

miR-191 modulates B-cell development and targets transcription factors E2A, Foxp1, and Egr1.
Blume J, Ziętara N, Witzlau K, Liu Y, Sanchez OO, Puchałka J, Winter SJ, Kunze-Schumacher H, Saran N, Düber S, Roy B, Weiss S, Klein C, Wurst W, Łyszkiewicz M, Krueger A.
Eur J Immunol. 2019; 49(1):121-132.

LAMTOR2 (p14) Controls B Cell Differentiation by Orchestrating Endosomal BCR Trafficking.
Łyszkiewicz M, Kotlarz D, Ziȩtara N, Brandes G, Diestelhorst J, Glage S, Hobeika E, Reth M, Huber LA, Krueger A, Klein C.
Front Immunol. 2019;10:497.

miR-181a/b-1 controls thymic selection of Treg cells and tunes their suppressive capacity.
Łyszkiewicz M, Winter SJ, Witzlau K, Föhse L, Brownlie R, Puchałka J, Verheyden NA, Kunze-Schumacher H, Imelmann E, Blume J, Raha S, Sekiya T, Yoshimura A, Frueh JT, Ullrich E, Huehn J, Weiss S, Gutierrez MG, Prinz I, Zamoyska R, Ziętara N, Krueger A.
PLoS Biol. 2019;17(3):e2006716.

Germline deletion reveals a non-essential role of the atypical MAPK6/ERK3.
Ronkina N, Schuster-Gossler K, Hansmann F, Kunze-Schumacher H, Sandrock I, Yakovleva T, Lafera J, Baumgärtner W, Krueger A, Prinz I, Gossler A, Kotlyarov A, Gaestel M.
Mol Cell Biol. 2019;39(6).

Chimeric antigen receptor-induced BCL11B suppression propagates NK-like cell development.
Maluski M, Ghosh A, Herbst J, Scholl V, Baumann R, Huehn J, Geffers R, Meyer J, Maul H, Eiz-Vesper B, Krueger A, Schambach A, van den Brink MR, Sauer MG.
J Clin Invest. 2019 Sep 3. pii: 126350.

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).
Cossarizza A et al. Eur J Immunol. 2019 Oct;49(10):1457-1973.

Development of Unconventional T Cells Controlled by MicroRNA.
Winter SJ, Krueger A.
Front Immunol. 2019 Oct 23;10:2520.


2018

Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b.
Glaesener S, Jaenke C, Habener A, Geffers R, Hagendorff P, Witzlau K, Imelmann E, Krueger A, Meyer-Bahlburg A.
PLoS One. 2018;13(2):e0192230.

Thymus Colonization: Who, How, How Many?
Krueger A.
Arch Immunol Ther Exp (Warsz). 2018;66(2):81-88. 

MicroRNA in T-Cell Development and T-Cell Mediated Acute Graft-Versus-Host Disease.
Koenecke C, Krueger A.
Front. Immunol., 07 May 2018.

miRNA miR-21 Is Largely Dispensable for Intrathymic T-Cell Development.
Kunze-Schumacher H, Winter SJ, Imelmann E, Krueger A.
Front. Immunol. 2018;9:2497.

Genetic models reveal origin, persistence and non-redundant functions of IL-17-producing γδ T cells.
Sandrock I, Reinhardt A, Ravens S, Binz C, Wilharm A, Martins J, Oberdörfer L, Tan L, Lienenklaus S, Zhang B, Naumann R, Zhuang Y, Krueger A, Förster R, Prinz I.
J Exp Med. 2018;215(12):3006-3018.


2017

T Cell Development by the Numbers.
Krueger A, Ziętara N, Łyszkiewicz M.
Trends Immunol. 2017;38:128-139.


2016

Overexpression of Vα14Jα18 TCR promotes development of iNKT cells in the absence of miR-181a/b-1.
Blume J, Zur Lage S, Witzlau K, Georgiev H, Weiss S, Łyszkiewicz M, Ziȩtara N, Krueger A. 
Immunol Cell Biol. 2016;94:741-6.

Establishing a murine xenograft-model for long-term analysis of factors inducing chromosomal instability in myelodysplastic syndrome: Pitfalls and successes.
Salari A, Thomay K, Himmler K, Vajen B, Schienke A, Hagedorn M, Ebersold J, Kreipe HH, Krüger A, Schambach A, Schlegelberger B, Göhring G.
Cancer Genet. 2016;209:258-66.

miR-181a Expression in Donor T Cells Modulates Graft-versus-Host Disease after Allogeneic Bone Marrow Transplantation.
Lee CW, Wohlan K, Dallmann I, Förster R, Ganser A, Krueger A, Scherr M, Eder M, Koenecke C.
J Immunol. 2016;196:3927-34.


2015

MicroRNA-181a/b-1 is not required for innate γδNKT effector cell development.
Sandrock I, Ziętara N, Łyszkiewicz M, Oberdörfer L, Witzlau K, Krueger A*, Prinz I*.
PLoS One. 2015;10:e0145010.
* Equal contribution.

Responsiveness of developing T cells to IL-7 signals is sustained by miR-17∼92.
Regelin M, Blume J, Pommerencke J, Vakilzadeh R, Witzlau K, Łyszkiewicz M, Ziętara N, Saran N, Schambach A, Krueger A.
J Immunol. 2015;195:4832-40.

Multicongenic fate mapping quantification of dynamics of thymus colonization.
Ziętara N, Łyszkiewicz M, Puchałka J, Witzlau K, Förster R, Pabst O, Prinz I, Krueger A.
J Exp Med. 2015; 212:1589-1601.

Limited niche availability suppresses murine intrathymic dendritic-cell development from non-committed progenitors.
Łyszkiewicz M, Ziętara N, Föhse L, Puchałka J, Diestelhorst J, Witzlau J, Prinz I, Schambach A,
Krueger A.
Blood. 2015;125:457-64.


2014

Resident CD4+ T cells accumulate in lymphoid organs after prolonged antigen exposure.
Ugur M, Schulz O, Menon MB, Krueger A, Pabst O.
Nat Commun. 2014;5:4821. doi:10.1038/ncomms5821.

B-cell modulation of dendritic-cell function: signals from the far side.
Ziętara N, Łyszkiewicz M, Krueger A*, Weiss S*.
Eur J Immunol. 2014;44:23-32.
*Co-corresponding authors.

S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development.
Braune EB, Schuster-Gossler K, Lyszkiewicz M, Serth K, Preusse K, Madlung J, Macek B, Krueger A, Gossler A.
Mol Cell Biol. 2014;34:1221-33.

Hypertrophy of infected Peyer's patches arises from global, interferon-receptor, and CD69-independent shutdown of lymphocyte egress.
Schulz O, Ugur M, Friedrichsen M, Radulovic K, Niess JH, Jalkanen S, Krueger A, Pabst O.
Mucosal Immunol. 2014;7:892-904.


2013

Critical role for miR-181a/b-1 in agonist selection of invariant natural killer T cells.
Ziętara N, Łyszkiewicz M, Witzlau K, Naumann R, Hurwitz R, Langemeier J, Bohne J, Sandrock I, Ballmaier M, Weiss S, Prinz I*, and Krueger A*.
Proc. Natl. Acad. Sci. USA. 2013;110:7407-7412.
* Equal contribution.

Immunoglobulins drive terminal maturation of splenic dendritic cells.
Ziętara N, Łyszkiewicz M, Puchałka J, Gutierrez MG, Pei G, Lienenklaus S, Hobeika E, Reth, M, Martins Dos Santos VAP, Krueger A*, and Weiss S*.
Proc. Natl. Acad. Sci. USA. 2013;110:2282-2287.
* Equal contribution.


2012

Development of interleukin-17 producing <gamma><delta> T cells is restricted to a functional embryonic wave.
Haas JD, Ravens S, Düber S, Chennupati V, Föhse L, Oberdörfer L, Naumann R, Weiß S, Krueger A, Förster R, Prinz I.
Immunity. 2012;37:48-59.

Extra-thymic physiological T lineage progenitor activity is exclusively confined to cells expressing either CD127, CD90, or high levels of CD117.
Saran N, Pommerencke J, Witzlau K, Regelin M, Krueger A.
PLoS One. 2012;7:e30864.


2011

ICOS-dependent stimulation of NKT cells by marginal zone B cells.
Ziętara N, Łyszkiewicz M, Krueger A, and Weiss S.
Eur J Immunol. 2011;41:3125-3134.

Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in ab and gd T cells.
Haas JD, Nistala K, Petermann F, Saran N, Chennupati V, Schmitz S, Korn T, Wedderburn LR, Förster R, Krueger A*, Prinz I*.
PLoS One. 2011;6:e20171.
* Equal contribution.

A missing link in thymic dendritic cell development.
Krueger A.
Eur J Immunol. 2011;41:2145-7.

Enforced expression of miR-125b affects myelopoiesis by targeting multiple signalling pathways.
Surdziel E, Cabanski M, Dallmann I, Łyszkiewicz M, Krueger A, Ganser A, Scherr M, Eder M.
Blood. 2011;117:4338-48.

Chemokine receptor CX3CR1 promotes dendritic cell development under steady-state conditions.
Łyszkiewicz M, Witzlau K, Pommerencke J, Krueger A.
Eur J Immunol. 2011;41:1256-65.


2010

CC chemokine receptor (CCR) 7 and 9 double-deficient hematopoietic progenitors are severely impaired in seeding the adult thymus.
Krueger A*, Willenzon S, Łyszkiewicz M, Kremmer E, Förster R.
Blood. 2010;115:1906-12.
* Corresponding author.

Multiple extra-thymic precursors contribute to T cell development with different kinetics.
Saran N, Łyszkiewicz M, Pommerencke J, Witzlau K, Vakilzadeh R, Ballmaier M, von Boehmer H, Krueger A.
Blood. 2010;115:1137-44.


2009

Discontinued Postnatal Thymocyte Development in Sphingosine 1-Phosphate-Lyase-Deficient Mice.
Weber C*, Krueger A*, Münk A, Bode C, Van Veldhoven PP, Gräler MH.
J Immunol. 2009;183:4292-301.
* Equal contribution.


2008

T cell receptor-instructed alphabeta versus gammadelta lineage commitment revealed by single-cell analysis.
Kreslavsky T, Garbe AI, Krueger A, von Boehmer H.
J Exp Med. 2008;205:1173-86.


2007

Identification of a T lineage-committed progenitor in adult blood.
Krueger A, von Boehmer H.
Immunity. 2007;26:105-16.

Dynamic visualization of thrombopoiesis within bone marrow.
Junt T, Schulze H, Chen Z, Massberg S, Goerge T, Krueger A, Wagner DD, Graf T, Italiano JE, Shivdasani RA, von Andrian UH.
Science. 2007;317:1767-70.


2006

Phenotypic plasticity of T cell progenitors upon exposure to Notch ligands.
Krueger A*, Garbe AI, von Boehmer H.
J Exp Med. 2006;203:1977-84.
* Corresponding author.

HTLV-1 Tax protects against CD95-mediated apoptosis by induction of the cellular FLICE-inhibitory protein (c-FLIP).
Krueger A*, Fas SC*, Giaisi M, Bleumink M, Merling A, Stumpf C, Baumann S, Holtkotte D, Bosch V, Krammer PH, Li-Weber M.
Blood. 2006;107:3933-9.
* Equal contribution.

The role of CAP3 in CD95 signaling: new insights into the mechanism of procaspase-8 activation.
Golks A, Brenner D, Schmitz I, Watzl C, Krueger A, Krammer PH, Lavrik IN.
Cell Death Differ. 2006;13:489-98.

Differential synergy of Notch and T cell receptor signaling determines alphabeta versus gammadelta lineage fate.
Garbe AI*, Krueger A*, Gounari F, Zuniga-Pflucker JC, von Boehmer H.
J Exp Med. 2006;203:1579-90.
* Equal contribution.

In vitro generated human memory-like T cells are CD95 type II cells and resistant towards CD95-mediated apoptosis.
Fas SC, Baumann S, Krueger A, Frey CR, Schulze-Bergkamen H, Brenner D, Stumpf C, Kappes K, Krammer PH.
Eur J Immunol. 2006;36:2894-903.

c-Myc mediates pre-TCR-induced proliferation but not developmental progression.
Dose M, Khan I, Guo Z, Kovalovsky D, Krueger A, von Boehmer H, Khazaie K, Gounari F.
Blood. 2006;108:2669-77.


2000-2005

FasL (CD95L/APO-1L) resistance of neurons mediated by phosphatidylinositol 3-kinase-Akt/protein kinase B-dependent expression of lifeguard/neuronal membrane protein 35.
Beier CP, Wischhusen J, Gleichmann M, Gerhardt E, Pekanovic A, Krueger A, Taylor V, Suter U, Krammer PH, Endres M, Weller M, Schulz JB.
J Neurosci. 2005;25:6765-74.

Glucocorticoids inhibit activation-induced cell death (AICD) via direct DNA-dependent repression of the CD95 ligand gene by a glucocorticoid receptor dimer.
Baumann S, Dostert A, Novac N, Bauer A, Schmid W, Fas SC, Krueger A, Heinzel T, Kirchhoff S, Schütz G, Krammer PH.
Blood. 2005;106:617-25.

Hepatocyte growth factor induces Mcl-1 in primary human hepatocytes and inhibits CD95-mediated apoptosis via Akt.
Schulze-Bergkamen H, Brenner D, Krueger A, Suess D, Fas SC, Frey CR, Dax A, Zink D, Büchler P, Müller M, Krammer PH.
Hepatology. 2004;39:645-54.

Resistance of short term activated T cells to CD95-mediated apoptosis correlates with de novo protein synthesis of c-FLIPshort.
Schmitz I, Weyd H, Krueger A, Baumann S, Fas SC, Krammer PH, Kirchhoff S.
J Immunol. 2004;172:2194-200.

Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs.
Ganten TM, Haas TL, Sykora J, Stahl H, Sprick MR, Fas SC, Krueger A, Weigand MA, Grosse-Wilde A, Stremmel W, Krammer PH, Walczak H.
Cell Death Differ. 2004;11:S86-96.

HDAC inhibitors trigger apoptosis in HPV-positive cells by inducing the E2F-p73 pathway.
Finzer P, Krueger A, Stöhr M, Brenner D, Soto U, Kuntzen C, Krammer PH, Rösl F.
Oncogene. 2004;23:4807-17.

Suramin inhibits death receptor-induced apoptosis in vitro and fulminant apoptotic liver damage in mice.
Eichhorst ST*, Krueger A*, Müerköster S, Fas SC, Golks A, Gruetzner U, Schubert L, Opelz C, Bilzer M, Gerbes AL, Krammer PH.
Nat Med. 2004;10:602-9.
* Equal contribution.

An IL-2-dependent switch between CD95 signaling pathways sensitizes primary human T cells toward CD95-mediated activation-induced cell death.
Schmitz I*, Krueger A*, Baumann S, Schulze-Bergkamen H, Krammer PH, Kirchhoff S.
J Immunol. 2003;171:2930-6.
* Equal contribution.

The role of CD95 in the regulation of peripheral T-cell apoptosis.
Krueger A, Fas SC, Baumann S, Krammer PH.
Immunol Rev. 2003;193:58-69.

The active caspase-8 heterotetramer is formed at the CD95 DISC.
Lavrik IN, Krueger A, Schmitz I, Baumann S, Weyd H, Krammer PH, Kirchhoff S.
Cell Death Differ. 2003;10:144-5.

An unexpected role for FosB in activation-induced cell death of T cells.
Baumann S, Hess J, Eichhorst ST, Krueger A, Angel P, Krammer PH, Kirchhoff S.
Oncogene. 2003;22:1333-9.

Specificity of anti-human CD95 (APO-1/Fas) antibodies.
Schmitz I*, Krueger A*, Baumann S, Kirchhoff S, Krammer PH.
Biochem Biophys Res Commun. 2002;297:459-62.
* Equal contribution. 

Viral IFN-regulatory factors inhibit activation-induced cell death via two positive regulatory IFN-regulatory factor 1-dependent domains in the CD95 ligand promoter.
Kirchhoff S, Sebens T, Baumann S, Krueger A, Zawatzky R, Li-Weber M, Meinl E, Neipel F, Fleckenstein B, Krammer PH.
J Immunol. 2002;168:1226-34.

Regulation of T cell apoptosis during the immune response.
Baumann S, Krueger A, Kirchhoff S, Krammer PH.
Curr Mol Med. 2002;2:257-72. 

Glutathione dependence of caspase-8 activation at the death-inducing signaling complex.
Hentze H, Schmitz I, Latta M, Krueger A, Krammer PH, Wendel A.
J Biol Chem. 2002;277:5588-95.

FLICE-inhibitory proteins: regulators of death receptor-mediated apoptosis.
Krueger A, Baumann S, Krammer PH, Kirchhoff S.
Mol Cell Biol. 2001;21:8247-54.

Cellular FLICE-inhibitory protein splice variants inhibit different steps of caspase-8 activation at the CD95 death-inducing signaling complex.
Krueger A, Schmitz I, Baumann S, Krammer PH, Kirchhoff S.
J Biol Chem. 2001;276:20633-40. 

Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes.
Eckhart L., Henry M, Santos-Beneit AM, Schmitz I, Krueger A, Fischer H, Bach J, Ban J, Kirchhoff S, Krammer PH, Mollinedo F, Tschachler E.
Biochem Biophys Res Commun. 2001;289:777-81.

TCR/CD3 Restimulation und CD28 Kostimulation induzieren die Expression von c‑FLIPshort und verhindern CD95 vermittelte Apoptose in T-Zellen.
Kirchhoff S, Krueger A, Baumann S, Krammer PH.
Immunologie Aktuell. 2001;1:117-8. (German). 

CD95/CD95 Ligand (Fas/FasL, APO-1/APO-1L).
Krammer PH, Krueger A.
In John Wiley and Sons, I. (ed.). 2001. Wiley Encyclopedia of Molecular Medicine.

Induction of apoptosis in 9-nitrocamptothecin-treated DU145 human prostate carcinoma cells correlates with de novo synthesis of CD95 and CD95 ligand and down-regulation of c-FLIP(short).
Chatterjee D, Schmitz I, Krueger A, Yeung K, Kirchhoff S, Krammer PH, Peter ME, Wyche JH,
Pantazis P.
Cancer Res. 2001;61:7148-54.

TCR-mediated up-regulation of c-FLIPshort correlates with resistance toward CD95-mediated apoptosis by blocking death-inducing signaling complex activity.
Kirchhoff S, Müller WW, Krueger A, Schmitz I, Krammer PH.
J Immunol. 2000;165:6293-300.