Plasmids and strains for use in C. elegans Optogenetics:

all exisiting in various expression plasmids for C. elegans, with different fluorescent proteins, and using different promoters (details available upon request). Several tools are also available from Addgene

• ChR2 WT: wild-type Channelrhodopsin
• ChR2(H134R): "stabilized"; "g.o.f." ChR2
• ChR2(H134R, T159C): improved conductance and expression
• ChR2(C128S): "slow" ChR2 (aka as "step-function opsin")
• C1V1-ET/ET: red-shifted action spectrum
• ChR2(L132C): Ca²⁺ transporting ChR (aka as "CatCh")
• Quint: Quintuple mutant, longest open state life time of any ChR reported

Halorhodopsin and other Hyperpolarizers:

• NpHR: Halorhodopsin from Natronomonas pharaonis
• Mac: Proton pump from Leptosphaeria maculans
• Arch: archaerhodopsin-3, proton pump from Halorubrum sodomense
• Anion Channelrhodopsins ACR1, ACR2, ZipACR

Photoactivated adenyly and guanylyl cyclases (PACs, PGCs)

• PAC from Euglena gracilis
• bPAC from Beggioatoa sp.
• CyclOp Guanylyl Cyclase opsin from Blastocladiella emersonii

Photosensitive Degron (PSD)

Photoactivated Botulinum Neurotoxin (paBoNT)

Vectors for single-cell expression using intersecting promoters

• cre recombinase
• loxP-STOP-LacZ-loxP::ChR2-SL2-GFP operon
• FLP recombinase
• FRT-mCherry-STOP-FRT::ChR2::YFP

Integrated transgenes

• zxIs3[punc-47::ChR2(H134R)::YFP; lin-15+]I: GABAergic neurons
• zxIs5[punc-17::ChR2(H134R)::YFP; lin-15+]X: cholinergic neurons
• zxIs6[punc-17::ChR2(H134R)::YFP; lin-15+]V: cholinergic neurons
• zxIs12[pF49H12.4::ChR2::mCherry; pF49H12.4::GFP]: PVD, AQR, tail neuron