Our phenotypic live-cell screens are performed with (modified, preferable adherent) cell lines and are image-based. Modifications include fluorescent proteins/reporters, stably expressed by the cell line. Treatment of choice may include arrayed media add-ons, arrayed knock-down of proteins (siRNA libraries), arrayed compound treatment (chemical libraries), as well as adding specific dyes required for assay specific analysis. Treatments may require the module Automated Liquid Handling that allows the handling of cells requiring biosafety level 1.
Technical Specifications
Automated liquid handling
- Multidrop Combi Reagent Dispenser (8-chanel-dispenser)
- Echo 550 automated liquid handler
FACS analysis & cell sorting
- Benchtop SH800SFP Cell Sorter
Phenotypic live-cell screening and High-Content Screening
- IncuCyte S3
- Yokogawa Quantitative Confocal Image Cytometer CQ1
Responsible: Dr. Alexandra Stolz