Gene perturbation is a powerful tool for dissecting genotype-to-phenotype relationships. Genome-wide and tailored CRISPR-Cas9 gRNAs and libraries have been used to identify essential and pathway-specific genes. This platform produces project-tailored reagents and libraries using 3Cs technology.
The quantitative phenotypic profiling of genes/sequences allows for the identification of i) single and synergistic gene essentiality and ii) novel pathways and complex components.
Technical Specifications
CRISPR-Cas9 gRNA library production
- Assembly of target gene list
- Design of 2-6 gRNAs per gene
- Cloning using 3Cs technology into lenti-, retro- or AVV-backbone
- Delivery as DNA, viral particles or transduced cells
Available genetic screen formats
- Drop-out
- Enrichment
- Drug synergizing or sensitizing
- Biochemical reporter/FACS enrichment/depletion
Responsible: Prof. Manuel Kaulich and Dr. Koraljka Husnjak