DARPins (Designed Ankyrin Repeat Proteins) are small (14-18 kDa), very stable proteins. Certain positions can be randomized to create by a combination of in vitro selection strategies (ribosome display and phage display) of high-affinity binders against folded domains/proteins. Due to the absence of disulfide bridges, DARPins can be used for intracellular applications, for example, as highly selective and affine binders or fused to E3 ligases as bio-PROTACS. In addition to DARPin libraries, we have Sybody (nanobody) libraries.
We can run selection experiments for DARPin and Sybodies to create high-affinity binders.
Technical Specifications
Generation of DARPins/Sybodies:
- Run ribosome display with DARPin libraries with different numbers of helical modules (1, 2 or 3) followed by two rounds of phage display
- Run ribosome display with Sybody libraries ( with short, medium or long loops) followed by two rounds of phage display
Responsible: Prof. Volker Dötsch, Dr. Katharina Holzhüter