Fluorescence Lifetime Imaging Microscopy
Fluorescence Lifetime Imaging Microscopy (FLIM) is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. Fluorescence lifetime imaging microscopes are classified in two main categories depending on the detection technique used, which are Time domain and Frequency domain. Our Time domain FLIM system is build up on Olympus reflected fluorescence microscope system and a Horiba instruments set-up with a time-correlated single photon counting (TCSPC) technique. X, Y, Z stage and CCD camera; TBX-PS detector power source; PicoBrite Picosecond laser diodes: wavelength 375 nm, 452 nm and 639 nm; Olympus BX 51: BF, 365 nm, 475 nm, 525 nm, and 640 nm BP filter.